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AB5622-I Anti-MAP-2

Anti-MAP-2, Cat. No. AB5622-I, is a highly specific rabbit polyclonal antibody that targets microtubule-associated protein 2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MAP-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Map2, Mtap2 UniProt Number: P15146
AB5622-I
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RICC, WB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5622-I
DescriptionAnti-MAP-2
Alternate Names
  • Mirotubule associated protein 2
Background InformationMicrotubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in Rat. MAPs are a family of proteins that bind to and stabilize microtubules. MAP4 is expressed ubiquitously while MAP1 and MAP2 are expressed primarily in neurons. MAP2 are cytoskeletal proteins that stabilize microtubules and prevents their depolymerization and may also have a stiffening effect on microtubules. MAP-2 plays an important role in brain neuron microtubule assembly. It also associates with neurofilaments and actin filaments and thereby guides interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. Four different isoforms of MAP2 have been described that bind to microtubules through a domain near the carboxyl terminus. MAP2 isoforms are expressed only in neuronal cells and specifically in the cell bodies and dendrites. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. However, some axons may also have small amounts of MAP-2. MAP-2 usually consists of MAP-2a and MAP-2b, ~280kDa each, and lower molecular weight MAP2c, which is present during neuronal differentiation in the developing nervous system. MAP-2 is a marker for neurons and is uniformly distributed throughout the cell when first expressed in cultured neurons. However, later it is selectively localized as dendritic development proceeds. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that results in detachment from microtubules, and their disassembly.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-MAP-2, Cat. No. AB5622-I, is a highly specific rabbit polyclonal antibody that targets microtubule-associated protein 2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in rat cerebral cortex and rat kidney tissue.

Western Blotting Analysis: 2 µg/mL from a representative lot detected MAP-2 in 10 µg of rat brain microsomal preparations.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected MAP-2 in rat primary cortex cells.
Biological Information
ImmunogenKLH-conjugated three peptides from N-and C-terminal regions of rat MAP-2.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects MAP2 in Mouse and Rat tissues.
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Map2
  • Mtap2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~280 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse cerebellum tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAP-2 in mouse cerebellum tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB5622-I 04054839176487