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MABS274 Sigma-AldrichAnti-LRP6, clone A59, Ectodomain Antibody

This Anti-LRP6 antibody, clone A59, Ectodomain is validated for use in flow cytometry, IP & ICC for the detection of LRP6.

Anti-LRP6, clone A59, Ectodomain Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: LRP6 UniProt Number: O75581

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	FC, IP, ICC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABS274
Description	Anti-LRP6, clone A59, Ectodomain Antibody
Alternate Names	Low-density lipoprotein receptor-related protein 6 LRP-6
Background Information	Low density lipoprotein receptor-related protein 6, also known as LRP6, is a member of the low density Lipoprotein receptor gene family (LDLR). LDLR are cell surface proteins. LRP6 plays a crucial role in embryonic development by functioning as a co-receptor for the Wnt signaling pathway. LRP6 is highly homologous and presents itself as a dimer in the cell membrane until it is phosphlorylated by Wnt. LRP6 has a high affinity with the Dickkopf (DKK) family of antagonists which, when bound, block Wnt. When LRP6 binds to Wnt it is phosphlorated at multiple sites, which results in Axin, a scaffolding protein, being recruited to the cell membrane and β-catenin activation. β-catenin prevents Wnt and LRP6 from binding. LRP6 is associated with autosomal dominant coronary artery disease type 2. Mutations resulting in the omission of the extracellular domain of LPR6 results in constant activity whereas carboxy intracellular domain exclusion has a negative effect, resulting in the blocking of Wnt signaling.

References

Product Information
Format	Purified
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-LRP6 antibody, clone A59, Ectodomain is validated for use in flow cytometry, IP & ICC for the detection of LRP6.
Key Applications	Flow Cytometry Immunoprecipitation Immunocytochemistry
Application Notes	Flow Cytometry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in Chimeric LRP6 expressing 293T cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.). Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated LRP6, Ectodomain from Wnt3a-treated HEK293T cell lysate (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.). Immunocytochemistry Analysis: A representative lot from an independent laboratory detected LRP6, Ectodomain in HeLa S3 cells (Yasui, N., et al. (2010). J Immunol Methods. 352(1-2):153-160.).

Biological Information
Immunogen	Purified protein corresponding to the ectodomain domain of human LRP6.
Epitope	Ectodomain Domain
Clone	A59
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_002327
Gene Symbol	LRP6
Purification Method	Protein G Purified
UniProt Number	O75581
Molecular Weight	180 kDa calculated

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Flow Cytometry in HEK293 cells. Flow Cytometry Analysis: 2 µg of this antibody detected LRP6, Ectodomain in 1X10E6 HEK293 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
MABS274	04053252955457

Documentation

Anti-LRP6, clone A59, Ectodomain Antibody SDS

Title
Safety Data Sheet (SDS)

Anti-LRP6, clone A59, Ectodomain Antibody Certificates of Analysis

Title	Lot Number
Anti-LRP6, clone A59, Ectodomain - Q2383199	Q2383199
Anti-LRP6, clone A59, Ectodomain Monoclonal Antibody	3049399
Anti-LRP6, clone A59, Ectodomain Monoclonal Antibody	2878134

References

Reference overview	Pub Med ID
Detection of endogenous LRP6 expressed on human cells by monoclonal antibodies specific for the native conformation. Yasui, Norihisa, et al. J. Immunol. Methods, 352: 153-60 (2010) 2010 Show Abstract LRP6 is a cell surface molecule that plays a critical role in the Wnt signaling pathway, and is implicated in numerous human diseases. Studies of cellular signaling mediated by LRP6 have relied on overexpression experiments, due to the lack of good monoclonal antibodies (mAbs) reactive with native LRP6 ectodomain. By using native recombinant LRP6 ectodomain fragment produced in mammalian expression system, we succeeded in developing a panel of anti-human LRP6 mAbs. Selected mAbs were capable of staining endogenous LRP6 on cell surface by using flow cytometry and immunofluorescence microscopy, and enriching detergent-solubilized LRP6 from cell lysate by immunoprecipitation.	19945460

Reference overview

Pub Med ID

Catalogue Number	Description
06-017	Anti-LRP6 Antibody, CT
MABS341	Anti-LRP6 Antibody, clone 1C10

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