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MABF294 Anti-LAG3 Antibody, clone 8C7.1

This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Anti-LAG3 Antibody, clone 8C7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: LAG3, FDC UniProt Number: P18627
MABF294
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCMCulture SupernatantMonoclonal Antibody
Description
Catalogue NumberMABF294
DescriptionAnti-LAG3 Antibody, clone 8C7.1
Alternate Names
  • Lymphocyte activation gene 3 protein
  • LAG-3
  • Protein FDC
  • CD223
  • LAG3
Background InformationLymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.
References
Product Information
FormatCulture Supernatant
PresentationMouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.
Biological Information
Immunogen Mouse Fc-tagged recombinant human LAG3 extracellular fragment.
Epitopeextracellular domain
Clone8C7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • LAG3
  • FDC
Purification MethodUnpurified
UniProt Number
Molecular Weight~60 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NK-92®c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92® cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF294 04055977274936