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MABN2299-100UG Anti-L1CAM/CD171 Antibody, clone S10.33

Anti-L1CAM/CD171, clone S10.33, Cat. No. MABN2299, is a rat monoclonal antibody that detects L1CAM/CD171 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.

MABN2299-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABN2299-100UG
DescriptionAnti-L1CAM/CD171 Antibody, clone S10.33
Alternate Names
  • Neural cell adhesion molecule L1
  • N-CAM-L1
  • NCAM-L1
  • CD171
Background InformationNeural cell adhesion molecule L1 (UniProt: P11627; also known as N-CAM-L1, NCAM-L1, CD171) is encoded by the L1cam (also known as Caml 1) gene (Gene ID: 16728) in murine species. L1CAM is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 20-1123), a transmembrane domain (aa 1124-1146), and a cytoplasmic domain (aa 1147-1260). The large extracellular domain contains six Ig-like C2 type domains and five fibronectin type III repeats. It serves as a cell recognition molecule within the nervous system that plays a critical role in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis during brain development. In the mature brain, it plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. Its expression is also observed in tumor vasculature. Tumor-bearing mice that lack L1CAM exhibit reduced angiogenesis and improved vascular stabilization that leads to reduced tumor growth and metastasis. These effects have been attributed to L1CAM-induced IL-6-mediated STAT3 phosphorylation and inhibition of IL-6/JAK/STAT signaling axis that prevents endothelial cell proliferation and migration. (Ref.: Magrini, A., et al. (2014). J. Clin. Invest. 124(10); 4335-4350; Maddaluno, L., et al. (2009). J. Exp. Med. Vol. 206(3); 623-635).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-L1CAM/CD171, clone S10.33, Cat. No. MABN2299, is a rat monoclonal antibody that detects L1CAM/CD171 and is tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Flow Cytometry
  • Immunofluorescence
Application NotesTested applications

Flow Cytometry Analysis: A representative lot detected L1CAM/CD171 in Flow Cytometry applications (Maddaluno, L., et al. (2009). J Exp Med. 206(3):623-35).

Immunohistochemistry Applications: A representative lot detected L1CAM/CD171 in Immunohistochemistry applications (Maddaluno, L., et al. (2009). J Exp Med. 206(3):623-35).

Immunofluorescence Analysis: A representative lot detected L1CAM/CD171 in Immunofluorescence applications (Maddaluno, L., et al. (2009). J Exp Med. 206(3):623-35; Magrini, E., et al. (2014). J Clin Invest. 124(10):4335-50).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRecombinant fragment corresponding to the entire extracellular domain of mouse L1CAM/CD171.
EpitopeExtracellular domain
CloneS10.33
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone S10.33 is a rat monoclonal antibody that detects L1CAM/CD171. It targets an epitope within the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • L1cam
Purification MethodProtein G purified
UniProt Number
Molecular Weight140.97 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in Neuro2A cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected L1CAM/CD171 in Neuro2A cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2299-100UG 04065270172549