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MABT1541-25UG Anti-JAM-A/F11R Antibody, clone CSTEM27

Anti-JAM-A/F11R, clone CSTEM27, Cat. No. MABT1541, is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.

MABT1541-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABT1541-25UG
DescriptionAnti-JAM-A/F11R Antibody, clone CSTEM27
Alternate Names
  • Junctional adhesion molecule A
  • JAM-A
  • Junctional adhesion molecule 1
  • JAM-1
  • Platelet F11 receptor
  • Platelet adhesion molecule 1
  • PAM-1
  • CD321
Background InformationJunctional adhesion molecule A (UniProt: Q9Y624; also known as JAM-A, Junctional adhesion molecule 1, JAM-1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321) is encoded by the F11R (also known as JAM1, JCAM) gene (Gene ID: 50848) in human. JAM-A/F11R is a single-pass type I membrane protein that is localized at tight junctions of both epithelial and endothelial cells. It is also expressed in circulating platelets and leukocytes. It is synthesized with a signal peptide (aa 1-27), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 28-238), a transmembrane domain (aa 239-259), and a cytoplasmic domain (aa 260-299). The extracellular domain contains a membrane distal VH-type Ig-like domain (D1; aa 28-125) and a membrane proximal C2-type Ig-like domain (D2; aa 135-228) that contains a single glycosylation site (Asn 185). The cytoplasmic domain includes two phosphorylation sites (Tyr 280 and Ser 284) and also has a type II PDZ domain-binding motif (aa 297-299). JAM-A/F11R plays a role in epithelial tight junction formation and also regulates monocyte transmigration involved in integrity of epithelial barrier. It participates in the regulation of various biological processes, including paracellular permeability, tight junction formation and maintenance, leukocyte trans-endothelial migration, epithelial-to-mesenchymal transition (EMT), angiogenesis, reovirus binding, and platelet activation. Its dysregulation may contribute to pathological consequences, such as cardiovascular diseases, inflammatory bowel disease, neurological disorders, and various cancer types. In cancer cells, it is not only associated with regulation of cell migration, but it also affects apoptosis, EMT, cancer stem cell maintenance, and cell proliferation. Down-regulation of JAM-A/F11R results in elevated cell migration, invasion, and metastasis in various breast cancer cell lines. Its higher levels have been linked to reduced cell migration ability of cancer cells. (Ref.: Czubak-Prowizor, K., et al. (2022). Mol. Cell. Biochem. 477(1); 79-98; Liu, X., et al. (2020). Nature. 586(7827); 101-107).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-JAM-A/F11R, clone CSTEM27, Cat. No. MABT1541, is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • ELISA
  • Immunocytochemistry
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected JAM-A/F11R in Flow Cytometry application (Liu, X., et al. (2017). Nat Methods. 14(11); 1055-1062; O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640; Liu, X., et al. (2020). Nature. 586(7827); 101-107).

ELISA Analysis: A representative lot detected JAM-A/F11R in ELISA application (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Immunocytochemistry Analysis: A representative lot detected JAM-A/F11R in Immunocytochemistry application (O Brien, C.M., et al. (2017). Stem Cells. 35(3); 626-640).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the extracellular domain of human Junctional adhesion molecule A (JAM-A/F11R).
EpitopeExtracellular domain
CloneCSTEM27
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone CSTEM27 is a mouse monoclonal antibody that detects Junctional adhesion molecule A (JAM-A/FR11). It targets an epitope within the extracellular domain.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • F11R
Purification MethodProtein G purified
UniProt Number
Molecular Weight~32 kDa observed; 32.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant Human JAM-A/F11R protein.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human JAM-A/F11R protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1541-25UG 04065268529096