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CP11L Anti-Integrin α₃ Mouse mAb (P1B5)

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Anti-Integrin α₃ Mouse mAb (P1B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP11L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
OverviewRecognizes the α3-subunit of the α3/β1 integrin complex protein in human mononuclear cells.
Catalogue NumberCP11L
Brand Family Calbiochem®
References
ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Cheresh, D.A. et al. 1990. Cell 57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
Hynes, R.O. 1987 Cell 48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Positive controlA2058 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.
Key Applications Cell Inhibition Assays
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Not Western Blot
Not Paraffin Sections
Application NotesCell Inhibition Assays (1-5 µg/ml, see application references)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
Application CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman mononuclear cells
ImmunogenHuman
CloneP1B5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CP11L 0

Documentation

Anti-Integrin α₃ Mouse mAb (P1B5) SDS

Title

Safety Data Sheet (SDS) 

Anti-Integrin α₃ Mouse mAb (P1B5) Certificates of Analysis

TitleLot Number
CP11L

References

Reference overview
Albelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Cheresh, D.A. et al. 1990. Cell 57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
Hynes, R.O. 1987 Cell 48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-March-2008 JSW
ApplicationCell Inhibition Assays (1-5 µg/ml, see application references)
Flow Cytometry (1-5 µg/ml)
Immunoblotting (not recommended)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.
BackgroundThe integrins are a family of cell surface glycoproteins that mediate both cell-cell adhesion and cell-substratum interactions. They are heterodimers consisting of noncovalently associated and subunits. Recently, it has been shown that there are six distinct subunits, and that some subunits may be associated with more than one βchain. The β1 or VLA family of integrins has the largest number of members and share the common β1 subunit. Studies have indicated that different integrins function in various combinations to mediate cell adhesion in different tissues. The α3/β1 integrin, also termed VLA3 or CD49c, has been shown to interact primarily with secreted, laminin-containing extracellular matrix.
HostMouse
Immunogen speciesHuman
Immunogenhuman mononuclear cells
CloneP1B5
IsotypeIgG₁
Specieshuman
Positive controlA2058 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsWill partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.
Toxicity Standard Handling
ReferencesAlbelda, S.M. and Buck, C.A. 1990. FASEB J. 4, 2868.
Cheresh, D.A. et al. 1990. Cell 57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem. 265, 823.
Vogel, B, et al. 1990. J. Biol. Chem. 265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol. 107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol. 3, 179.
Hynes, R.O. 1987 Cell 48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873.
Application referencesOriginal Clone Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol. 105, 1873. Cell Inhibition Assays Carter, W.G. et al. 1990. J. Cell Biol. 110, 1387.