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ABF1820-25UL Anti-Influenza A nucleoprotein

Anti-Influenza A nucleoprotein, Cat. No. ABF1820, is a rabbit polyclonal antibody that detects influenza virus nucleoprotein and has been tested for use in Immunofluorescence and Western Blotting.

Anti-Influenza A nucleoprotein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: NP UniProt Number: Q9WLG4
ABF1820-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VirusIF, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberABF1820-25UL
DescriptionAnti-Influenza A nucleoprotein
Alternate Names
  • Nucleoprotein
  • Nucleocapsid protein
  • Protein N
Background InformationNucleoprotein (UniProt: Q9WLG4; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene in Influenza A virus (strain A/Victoria/3/1975 H3N2). The genomes of all negative-strand RNA viruses are coated by the viral nucleoprotein. Together with the viral RNA-dependent RNA polymerase, these ribonucleoprotein complexes form the minimal functional unit for all viral transcription and replication. Influenza A virus has a negative-sense RNA genome that encodes a single-strand RNA-binding nucleoprotein. The primary function of the nucleoprotein is to encapsidate the virus genome for the purposes of RNA transcription, replication, and packaging. It is considered to be a critical factor in the viral infectious cycle in switching influenza virus RNA synthesis from transcription mode to replication mode. It also functions as a key adapter molecule between virus and host cell processes. It achieves this through its ability to interact with a wide variety of viral and cellular macromolecules, including RNA, itself, two subunits of the viral RNA-dependent RNA polymerase and the viral matrix protein. Nuceloprotein can also interact with cellular polypeptides, including actin, components of the nuclear import and export apparatus and a nuclear RNA helicase. Influenza A nucleoprotein has an unconventional nuclear localization signal (NLS) (aa 1-18) and a bipartite NLS (aa 1980216). (Ref.: Portela, A., and Digard, P. (2002). J. Gen. Virol. 83(4); 723-34; de Lucas, S., et al. (2010). J. Virol. 84(15); 7603-7612).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum in 0.05% sodium azide.
Applications
ApplicationAnti-Influenza A nucleoprotein, Cat. No. ABF1820, is a rabbit polyclonal antibody that detects influenza virus nucleoprotein and has been tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells were infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

Western Blotting Analysis: A representative lot detected Influenza A nucleoprotein in Western Blotting applications (Jorba, N., et. al. (2009). PLoS Pathog. 5(5):e1000462; Lukarsk, M., et. al. (2017). Nature. 541(7635):117-121; Tripathi, S., et. al. (2015). Cell Host Microbe. 18(6):723-35).

Immunofluorescence Analysis: A representative lot detected Influenza A nucleoprotein in Immunofluorescence applications (Marcos-Villar, L., et. al. (2018). Sci Rep. 8(1):1230; Rodriguez-Fandsen, A., et. al. (2016). Sci Rep. 6:20744).
Biological Information
ImmunogenHig-tagged full length recombinant nucleoprotein from Influenza A virus.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects influenza A virus nucleoprotein in virus infected cells.
IsotypeIgG
Species Reactivity
  • Virus
Species Reactivity NoteInfluenza A Virus.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NP
Purification MethodUnpurified
UniProt Number
Molecular Weight~56 kDa observed; 56.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cells infected with A/Victoria/3/75 strain of influenza virus.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Influenza A nucleoprotein in A549 cells infected with A/Victoria/3/75 strain of influenza virus.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF1820-25UL 04054839540158