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AP1153 Anti-IL12RB2 Mouse mAb (2H6)

AP1153
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the IL12RB2 protein.
Catalogue NumberAP1153
Brand Family Calbiochem®
SynonymsAnti-Interleukin 12 Receptor β2
Application Data
Detection of human IL12RB2 by immunoblotting. Sample: Partial recombinant IL12RB2 human protein. Primary antibody: Anti-IL12RB2 Mouse mAb (2H6) (Cat. No. AP1153) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 105-215 of human IL12RB2, expressed as a GST fusion protein
Clone2H6
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1153 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-October-2010 JLN
SynonymsAnti-Interleukin 12 Receptor β2
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human IL12RB2 by immunoblotting. Sample: Partial recombinant IL12RB2 human protein. Primary antibody: Anti-IL12RB2 Mouse mAb (2H6) (Cat. No. AP1153) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the IL12RB2 protein.
BackgroundIL12RB2 is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of IL12RB2 and IL12RB1 proteins is shown to lead to the formation of high-affinity IL12 binding sites and the reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 105-215 of human IL12RB2, expressed as a GST fusion protein
Clone2H6
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review