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06-1085-I Anti-IL-7Ra (CD127), extracellular Antibody

This Anti-IL-7Ra (CD127), extracellular Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of IL-7Ra (CD127), extracellular.

Anti-IL-7Ra (CD127), extracellular Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: IL7R UniProt Number: P16871
06-1085-I
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number06-1085-I
DescriptionAnti-IL-7Ra (CD127), extracellular Antibody
Alternate Names
  • Interleukin-7 receptor subunit alpha
  • IL-7 receptor subunit alpha
  • IL-7R subunit alpha
  • IL-7R-alpha
  • IL-7RA
  • CDw127
  • CD127
Background InformationThe R34-34 antibody is specific for human CD127, a cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity. The R34-34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM), whereas certain populations of Foxp3+ /CD25+ T cells lacking CD127 have been associated with impaired suppressive function (Bikker et al. 2012. Rheumatology, 51: 996-1005)
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-IL-7Ra (CD127), extracellular Antibody is validated for use in Western Blotting and Flow Cytometry for the detection of IL-7Ra (CD127), extracellular.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected IL-7Ra (CD127) in 10 µg of human spleen tissue lysate.
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected IL-7Ra (CD127) in 1X10E6 human peripheral blood mononuclear cells (PBMCs).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the extracellular domain of human IL-7Ra (CD127).
EpitopeExtracellular domain
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IL7R
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~70 kDa observed. This protien is highly and variably glycosylated, and may vary from the calculated molecular weight of ~52kDa (Vranjkovi, A., et al., International Immunology, Vol. 19, No. 12, pp. 1329–1339). Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-7Ra (CD127) in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-1085-I 04055977112481