Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

04-1585 Anti-IL-18 Antibody, clone 12E7.1

Anti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.

Anti-IL-18 Antibody, clone 12E7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Cytokines & Cytokine Receptors Gene Symbol: IL-18, IL18, IGIF, IL-18, IL1F4, IL-1g UniProt Number: Q14116
04-1585
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIHC, FCMPurifiedMonoclonal Antibody
Description
Catalogue Number04-1585
DescriptionAnti-IL-18 Antibody, clone 12E7.1
Alternate Names
  • interleukin 18 (interferon-gamma-inducing factor)
  • IL-1 gamma
  • IFN-gamma-inducing factor
  • iboctadekin
  • interleukin-18
  • Interleukin-1 gamma
  • Iboctadekin
  • Interferon gamma-inducing factor
Background InformationInterleukin-18 (UniProt: Q14116; also known as IL-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma) is encoded by the IL18 (also known as IGIF, IL1F4) gene (Gene ID: 3606) in human. IL-18 is a pro-inflammatory cytokine that is shown to evoke both innate and acquired responses. It is synthesized with a propeptide (aa 1-36), which is subsequently cleaved off by caspases 1 and 4 to generate the active mature form. IL-18 is reported to be constitutively expressed in monocytes, macrophages, epithelial cells, osteoblasts, keratinocytes, and synovial fibroblasts. Its expression has also been reported in adrenal cortical cells. Mature IL-18 acts by binding to the IL-18 receptor a (IL-18a) to form a low affinity binary complex that recruits IL-18b to form a high affinity complex that leads to the activation of NF-kB and MAP kinase pathways. IL-18 can synergize with IL-12 to induce interferon-g synthesis from T-helper 1 and natural killer cells.
References
Product Information
FormatPurified
Control
  • Mouse splenocytes
PresentationPurified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-IL-18 Antibody, clone 12E7.1 is an antibody against IL-18 for use in IH, FC.
Key Applications
  • Immunohistochemistry
  • Flow Cytometry
Application NotesImmunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in human tonsil tissue.

Immunohistochemistry Analysis: 1:250 from a previous lot detected IL-18 in inflamed skin and normal human spleen tissues.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human IL-18.
Clone12E7.1
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgMκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a proinflammatory cytokine. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. [provided by RefSeq].
Gene Symbol
  • IL-18
  • IL18
  • IGIF
  • IL-18
  • IL1F4
  • IL-1g
UniProt Number
UniProt SummaryFUNCTION: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

SUBCELLULAR LOCATION: Secreted.

SEQUENCE SIMILARITIES: Belongs to the IL-1 family.
Molecular Weight~24 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse splenocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected IL-18 in mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1585 04053252629402

Documentation

Anti-IL-18 Antibody, clone 12E7.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-IL-18 Antibody, clone 12E7.1 Certificates of Analysis

TitleLot Number
Anti-IL-18, Clone 12E7.1 - 4068662 4068662
Anti-IL-18, clone 12E7.1 - 2142592 2142592
Anti-IL-18, clone 12E7.1 - 2430982 2430982
Anti-IL-18, clone 12E7.1 - 2201511 2201511
Anti-IL-18, clone 12E7.1 - 2283350 2283350
Anti-IL-18, clone 12E7.1 - 2332581 2332581
Anti-IL-18, clone 12E7.1 - 3234492 3234492
Anti-IL-18, clone 12E7.1 - 3425584 3425584
Anti-IL-18, clone 12E7.1 - 3750062 3750062
Anti-IL-18, clone 12E7.1 - 3876260 3876260
Show more