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MABE1064-25UG Anti-HsSAS-6 Antibody, clone 91.390.21

Detect SASS6 using this mouse monoclonal Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064-25UG, validated for use in Immunofluorescence, Immunoprecipitation and Western Blotting.

Anti-HsSAS-6 Antibody, clone 91.390.21 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: SASS6, SAS6 UniProt Number: Q6UVJ0
MABE1064-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1064-25UG
DescriptionAnti-HsSAS-6 Antibody, clone 91.390.21
Alternate Names
  • Spindle assembly abnormal protein 6 homolog
Background InformationSpindle assembly abnormal protein 6 homolog (UniProt: Q6UVJ0; also known as HsSAS-6) is encoded by the SASS6 (also known as SAS6) gene (Gene ID: 163786) in human. HsSAS-6 is a central scaffolding component of the centrioles that ensure their 9-fold symmetry. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. It is required for centrosome biogenesis and duplication and is essential for both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multi-ciliated cells. It contains a 35 nM long coiled-coil domain that mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring. Over-expression of SASS6 gene results in excess foci-bearing centriolar markers. Mutations in SASS6 are known to cause microcephaly 14 that is characterized by small head circumference, markedly reduced brain weight and disproportionately small cerebral cortex.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect SASS6 using this mouse monoclonal Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064-25UG, validated for use in Immunofluorescence, Immunoprecipitation and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected HsSAS-6 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A representative lot detected HsSAS-6 in RNAi in U2OS cell lysates (Courtesy of Professor Dr. Erich Nigg, Ph.D; Biozentrum, University of Basel, Switzerland).
Immunofluorescence Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunofluorescence (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).
Immunofluorescence Analysis: A representative lot detected HsSAS-6 in Immunofluorescence applications (Kleylein-Sohn, J. et. al. (2007). Dev Cell. 13(2):190-202 ; Sonnen, K..,et. al. (2012). Biol Open 1(10):965-76; Arquint, C., et al. (2012). J Cell Sci. 125(Pt 5):1342-52 ).
Immunoprecipitation Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunoprecipitation (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to 254 amino acids from the C-terminal half of human Spindle assembly abnormal protein 6 homolog (HsSAS-6).
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 91.390.21 detects HsSAS-6 protein in human cells. It targets an epitope with in 254 amino acids in the C-terminal half.
IsotypeIgG2b
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SASS6
  • SAS6
Purification MethodProtein G purified
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected HsSAS-6 in 10 µg of  U2OS cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1064-25UG 04054839437946