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ABE1791-100UL Anti-Histone H3Q5ser

ABE1791-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberABE1791-100UL
DescriptionAnti-Histone H3Q5ser
Alternate Names
  • H3Q5Ser
Background InformationHistone H3.1 UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication. Histone 3.1 can also undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. This H3Q5ser antibody only detects signal when the nucleosomes have been transamidated with serotonin and does not react with unmodified version.
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Histone H3Q5ser, Cat. No. ABE1791, is a highly specific rabbit polyclonal antibody that targets Histone H3.1 serotonylated at glutamine 5 position and has been tested for use in ChIP-seq, Dot Blot, and Western Blotting.
Key Applications
  • ChIP-seq
  • Dot Blot
  • Western Blotting
Application NotesWestern Blotting Analysis: A representaive lot detected Histone H3Q5ser in Western Blotting applications (Farrelly, L.A., et. al. (2019). Nature. 567(7749):535-539).

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Histone H3Q5ser in acid extracted histones from whole mouse brain (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

Dot Blot Analysis: Unmodified and various modified Histone peptides were probed with Histone H3Q5ser (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).

ChIP-seq Analysis: A representative lot detected Histone H3Q5ser in DNA input (differentiated RN46A-B14 cells) (Courtesy of Dr. Ian Maze, Icahn School of Medicine at Mount Sinai, New York).


Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3.1 serotonylated on glutamine 5.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Histone H3.1 serotonylated at glutamine 5 position.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H3
  • H3FT
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~16 kDa observed; 15.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Dot Blot on H3Q5-Serotonyl peptide.



Dot Blot Analysis: A 1:500 dilution of this antibody detected Histone H3Q5serH3Q5-Serotonyl peptide, but not the unmodified H3(1-10)-control peptide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
ABE1791-100UL 04054839010385