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MABE1885-100UG Anti-Histone H1 Antibody, clone 1G6

Anti-Histone H1, clone 1G6 Antibody, Cat. No. MABE1885, is a highly specific mouse monoclonal antibody that targets Drosophila Histone H1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting.

Anti-Histone H1 Antibody, clone 1G6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: His 1 UniProt Number: P02255
MABE1885-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
DrChIP, ChIP-seq, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1885-100UG
DescriptionAnti-Histone H1 Antibody, clone 1G6
Alternate Names
  • H1
Background InformationHistone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species. Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation. The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites. Histone H1 is essential for the condensation of nucleosome chains into higher-order structures. In metazoans, H1 histones are smaller in size with about 200 amino acids. They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail. The central globular domain is shown to be highly conserved among all H1 subtypes. Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes. Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding. In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage. Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs). Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis. (Ref.: Hergeth, SP., and Schneider, R (2015). EMBO Rep. 16(11); 1439-1453).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Histone H1, clone 1G6 Antibody, Cat. No. MABE1885, is a highly specific mouse monoclonal antibody that targets Drosophila Histone H1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), ChIP-seq, Immunoprecipitation, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
  • Immunoprecipitation
  • Western Blotting
Application NotesChIP-seq Analysis: A representative lot detected Histone H1 in ChIP-seq applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H1 in Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated Histone H1 in Chromatin Immunoprecipitation applications (Iwasaki, Y.W., et. al. (2016). Mol Cell. 63(3):408-19).
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 148 amino acids from the C-terminal half of Drosophila Histone H1.
EpitopeC-terminus
Clone1G6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1G6 is a mouse monoclonal antibody that detects Histone H1 in Drosophila species.
IsotypeIgG1κ
Species Reactivity
  • Drosophila
Species Reactivity NoteDrosophila.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • His 1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~35 kDa observed; 26.36 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Drosophila S2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Histone H1 in Drosophila S2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1885-100UG 04054839589591