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CB1029 Anti-HAMP Mouse mAb (1F9)

Anti-HAMP Mouse mAb (1F9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Hepcidin Antimicrobial Peptide

CB1029
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the HAMP protein.
Catalogue NumberCB1029
Brand Family Calbiochem®
SynonymsAnti-Hepcidin Antimicrobial Peptide
Application Data
Detection of human HAMP by immunoblotting. Sample: Partial recombinant HAMP. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HAMP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (3 µg/ml). Detection: colorimentric (DAB).
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHuman liver tissue
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 25-85 of human HAMP, expressed as a GST fusion protein
ImmunogenNA
Clone1F9
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1029 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-October-2010 RFH
SynonymsAnti-Hepcidin Antimicrobial Peptide
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human HAMP by immunoblotting. Sample: Partial recombinant HAMP. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human HAMP by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human liver tissue. Primary antibody: Anti-HAMP Mouse mAb (1F9) (Cat. No. CB1029) (3 µg/ml). Detection: colorimentric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the HAMP protein.
BackgroundThe protein is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. Mutations in this protein cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that result in cardiomyopathy, cirrhosis, and endocrine failure.
HostMouse
Immunogen speciesNA
ImmunogenA recombinant polypeptide corresponding to amino acids 25-85 of human HAMP, expressed as a GST fusion protein
Clone1F9
IsotypeIgG₁
Specieshuman
Positive controlHuman liver tissue
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review