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MABF3164-100UG Anti-H2-Kb Antibody, clone 25D1.16

Anti-H2-Kb, clone 25D1.16, Cat. No. MABF3164, is a mouse monoclonal antibody that detects H2-Kb and is tested for use in Electron Microscopy, Flow Cytometry, Immunocytochemistry, and Western Blotting.

MABF3164-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3164-100UG
DescriptionAnti-H2-Kb Antibody, clone 25D1.16
Alternate Names
  • H-2 class I histocompatibility antigen
  • K-B alpha chain
Background InformationH-2 class I histocompatibility antigen, K-B alpha chain (UniProt: P01901; also known as H-2K(B)) is encoded by the H2-K1 (also known as H2-K) gene (Gene ID: 14972) in murine species. Major histocompatibility complexes (MHCs) are cell surface molecules that play a major part of the immune system in all vertebrates by determining histocompatibility. The main function of MHCs is to bind and present peptide fragments derived from pathogens on the surface of antigen presenting cells. MHCs are divided into two major classes, class I MHCs are heterodimers composed of a transmembrane -chain and a common extracellular β-chain. The -chain contains three domains ( 1, 2, and 3), with the 1 domain mediating interaction with the β2 subunit. The 1 and 2 domains form the antigen-binding groove. H2-Kb, a member of the MHV class I family, is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 22-305), a transmembrane domain (aa 306-328), and a cytoplasmic domain (aa 329-369). It is involved in the presentation of foreign antigens to the immune system, particularly to T cells expressing CD3/TCR and CD8. The peptide-binding site of H2-Kb contains a deep C pocket, which is critical for peptide binding, as it accepts the anchor phenylalanine or tyrosine residue located in the middle of H2-Kb binding peptides. It predominantly binds octameric peptides. Clone 25D1.16 is shown to be specific for the ovalbumin-derived peptide SIINFEKL (OVA257-264) bound to the MHC class I molecule H-2Kb. This clone reacts with cells pulsed with SIINFEKL and with cells pulsed with V-OVA. (Ref.: Ferez, M., et al. (2014). J. Immunol. 192; 52-58; Porgador, A., et al. (1997). Immunity. 6; 715-726).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-H2-Kb, clone 25D1.16, Cat. No. MABF3164, is a mouse monoclonal antibody that detects H2-Kb and is tested for use in Electron Microscopy, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Electron Microscopy
  • Immunocytochemistry
  • Western Blotting
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected H2-Kb in Immunocytochemistry applications (Porgador, A., et al. (1997). Immunity. 6(6)715-26; Ferez, M., et al. (2014). J Immunol. 192(1):52-8).

Electron Microscopy: A representative lot detected H2-Kb in Electron Microscopy applications (Ferez, M., et al. (2014). J Immunol. 192(1):52-8).

Western Blotting Analysis: A representative lot detected H2-Kb in Western Blotting applications (Hu, C.F., et al. (2021). Vaccines (Basel). 9(3):278).

Flow Cytometry Analysis: A representative lot detected H2-Kb in Flow Cytometry applications (Porgador, A., et al. (1997). Immunity. 6(6)715-26; Ferez, M., et al. (2014). J Immunol. 192(1):52-8).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenRMA-S cells, preincubated with chicken ovalbumin peptide (SIINFEKL).
EpitopeUnknown
Clone25D1.16
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 25D1.16 is a mouse monoclonal antibody that detects H-2 class I histocompatibility antigen, K-B alpha chain (H-2K(B).
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2-K1
Purification MethodProtein G purified
UniProt Number
Molecular Weight41.3 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Mouse splenocytes pulsed with SIINFEKL peptide.

Flow Cytometry Analysis: 1.0 µg of this antibody detected H2-kb in one million Mouse splenocytes pulsed with SIINFEKL peptide (50 M, for 2 h).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3164-100UG 04065270321596