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368673 Anti-Grp78 (645-654) Rabbit pAb

368673
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes the ~78 kDa Grp78 protein.
Catalogue Number368673
Brand Family Calbiochem®
SynonymsAnti-BiP
Application Data
Detection of mouse Grp78 by immunoblotting. Samples: Molecular weight marker (lane 1), Grp78 (BiP) recombinant protein (SPP-765) (lane 2), whole cell lysate from CHO-K1 cells (lane 3), and extract from mouse liver microsome (lane 4). Primary antibody: Anti-Grp78 (645-654) Rabbit pAb (Cat. No. 368673) (1:1000). Detection: chemiluminescence.
References
ReferencesTing, J., and Lee, A.S. 1989. DNA 7, 275.
Munro, S., and Pelham, H.R.B. 1987. Cell 48, 899.
Warren, G. 1987. Nature 327, 17.
Munro, S., and Pelham, H.R.B. 1986. Cell 46, 291.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
Application CommentsThe immunogen sequence is identical to the corresponding sequence in mouse and hamster Gfp78, but cross-reactivity has not been tested. To reduce background by immunoblotting, we recommend blocking with 10% (w/v) skim milk powder in PBS, 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide (EEDTSEKDEL) corresponding to amino acids 645-654 of rat Grp78, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
368673 0