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MABS2302-100UG Anti-GalNAc-T5 Antibody, clone 5F11

Anti-GalNAc-T5, clone 5F11, Cat. No. MABS2302, is a mouse monoclonal antibody that detects GalNAc-T5 and is tested for use in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.

MABS2302-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS2302-100UG
DescriptionAnti-GalNAc-T5 Antibody, clone 5F11
Alternate Names
  • Polypeptide N-acetylgalactosaminyltransferase 5
  • EC:2.4.1.41
  • Polypeptide GalNAc transferase 5
  • GalNAc-T5
  • pp-GaNTase 5
  • Protein-UDP acetylgalactosaminyltransferase 5
  • UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5
Background InformationPolypeptide N-acetylgalactosaminyltransferase 5 (UniProt: Q7Z7M9; also known as EC:2.4.1.41, Polypeptide GalNAc transferase 5, GalNAc-T5, pp-GaNTase 5, Protein-UDP acetylgalactosaminyltransferase 5, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 5) is encoded by the GALNT5 gene (Gene ID: 11227) in human. Complex carbohydrates synthesis in mammals involves over 200 distinct glycosyltransferases that differ in their cellular expression and their glycosylation capacity. A majority of them are localized in the endoplasmic reticulum or Golgi and their specific subcellular localization is believed to be distributed in the secretory pathway according to their sequential role in the glycosylation process. GalNAc-T5 is a single-pass type II membrane glycoprotein with a cytoplasmic domain (aa 1-12), a transmembrane domain (aa 13-35), and a lumenal domain (aa 36-940). It catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. It displays activity toward EA2 peptide substrates but has a weak activity toward Muc2 or Muc1b substrates. GalNAc-T5 has two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif) that is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif) that is involved in catalytic reaction and UDP-Gal binding. It also contains a ricin B-type lectin domain (aa 804-935) that binds to GalNAc and contributes to the glycopeptide specificity. Strong expression of GalNAc-T5 has been reported in normal gastric epithelium and in gastric cancer cells. However, its expression in normal gastric tissues is limited to a perinuclear Golgi-like localization and it is expressed throughout the cytoplasm of gastric cancer cells. (Ref.: Steentoft, C., et al. (2019). Glycobiology. 29(9); 645-656; Campos, D., et al. (2015). Mol. Cell. Proteomics. 14(6); 1616-1629).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-GalNAc-T5, clone 5F11, Cat. No. MABS2302, is a mouse monoclonal antibody that detects GalNAc-T5 and is tested for use in Immunocytochemistry, Immunofluorescence, and Immunohistochemistry.
Key Applications
  • Immunohistochemistry
  • Immunofluorescence
  • Immunocytochemistry
Application NotesTested Applications

Immunocytochemistry Analysis: A representative lot detected GalNAc-T5 in Immunocytochemistry application (Campos, D., et al. (2015). Mol Cell Proteomics.14(6);1616-29; Norden, R., et al. (2015). J Biol Chem. 290(8); 5078-5091; Steentoft, C., et al. (2019). Glycobiology. 29(9); 645-656).

Immunofluorescence Analysis: A representative lot detected GalNAc-T5 in Immunofluorescence application (Campos, D., et al. (2015). Mol Cell Proteomics. 14(6); 1616-29; Norden, R., et al. (2015). J Biol Chem. 290(8); 5078-5091).

Immunohistochemistry Application : A representative lot detected GalNAc-T5 in Immunohistochemistry application (Campos, D., et al. (2015). Mol Cell Proteomics. 14(6); 1616-29.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged, full-length recombinant human GalNAc-T5 expressed in Sf9 insect cells.
EpitopeUnknown
Clone5F11
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5F11 is a mouse monoclonal antibody that detects GalNAc transferase 5 (GalNAc-T5).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • GALNT5
Purification MethodProtein G purified
UniProt Number
Molecular Weight106.27 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human colon tissue sections.



Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GalNAc-T5 in human colon tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2302-100UG 04065268529140