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ST1629 Anti-GNA13 Mouse mAb (6F6-B5)

Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting.

Anti-GNA13 Mouse mAb (6F6-B5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13

ST1629
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1629-100UG 100 μg
Description
OverviewRecognizes the ~40-45 kDa GNA13 protein in HepG2 cells.
Catalogue NumberST1629
Brand Family Calbiochem®
SynonymsAnti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13
Application Data
Detection of human GNA13 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-GNA13 Mouse mAb (6F6-B5) (Cat. No. ST1629) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Negative control293T cells
Positive controlHepG2 cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
Clone6F6-B5
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1629-100UG 04055977223729

Documentation

Anti-GNA13 Mouse mAb (6F6-B5) SDS

Title

Safety Data Sheet (SDS) 

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-November-2010 RFH
SynonymsAnti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human GNA13 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-GNA13 Mouse mAb (6F6-B5) (Cat. No. ST1629) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~40-45 kDa GNA13 protein.
BackgroundHeterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an α-, β- and γ-subunit and is bound to GDP in the 'off' state. Ligand binding to a 7-TM receptor causes a conformational change in the receptor, which detaches the G protein. This reaction switches the G protein to an 'on' state, allowing GTP to displace GDP, thus Gα can activate second messenger signaling cascades. There are several types of Gα proteins; Gαs and Gαi bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively. Gαq/11 stimulates PLCβ which catalyzes the hydroylsis of PIP2 to the second messengers IP3 and DAG. Gv12/13 are involved in Rho family GTPase signaling. In addition, some Gβγ subunits have active functions; Gβv coupled to H1 receptors can activate PLA2 and Gβγ coupled to M1 receptors can activate KIR channels. Rapid termination of the cellular response is required once the concentration of the stimulating ligand decreases. GTP bound to Gα is hydrolyzed rapidly to GDP, Gα is converted to its inactive form and re-associates with the β- and γ-subunits returning to the 'off' state.
HostMouse
ImmunogenFull-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein
Clone6F6-B5
IsotypeIgG₁
Specieshuman
Positive controlHepG2 cells
Negative control293T cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review