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DR1083 Anti-GCLM Mouse mAb (2B8)

Anti-GCLM Mouse mAb (2B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate-Cysteine Ligase Modifier Subunit

DR1083
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~30 kDa GCLM protein in HeLa cells.
Catalogue NumberDR1083
Brand Family Calbiochem®
SynonymsAnti-Glutamate-Cysteine Ligase Modifier Subunit
Application Data
Detection of human GCLM by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GCLM Mouse mAb (2B8) (Cat. No. DR1083) (1-5 µg/ml). Detection: chemiluminescence.
References
ReferencesBalamurugan, A,N., et al. 2009. Pancreas 38, 454.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenFull-length, recombinant, human GCLM (aa 1-275), expressed as a GST fusion protein
Clone2B8
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DR1083 0

Documentation

References

Reference overview
Balamurugan, A,N., et al. 2009. Pancreas 38, 454.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-November-2010 RFH
SynonymsAnti-Glutamate-Cysteine Ligase Modifier Subunit
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human GCLM by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GCLM Mouse mAb (2B8) (Cat. No. DR1083) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~30 kDa GCLM protein.
BackgroundGlutamate-cysteine ligase, also known as γ-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
HostMouse
ImmunogenFull-length, recombinant, human GCLM (aa 1-275), expressed as a GST fusion protein
Clone2B8
IsotypeIgG2a
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesBalamurugan, A,N., et al. 2009. Pancreas 38, 454.