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PC168 Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb

This Anti-Estrogen Receptor β Rabbit pAb is validated for use in Frozen Sections, Gel Shift, WB, Paraffin Sections for the detection of Estrogen Receptor β.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-ER β

PC168
  
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody
Description
OverviewRecognizes the ~60 kDa estrogen receptor β protein in overexpressing cell lines and breast carcinoma tissue. Does not cross-react with estrogen receptor α.
Catalogue NumberPC168
Brand Family Calbiochem®
SynonymsAnti-ER β
Application Data
Detection of mouse estrogen receptor β by immunoblotting. Samples: I-11.15 cells (lane A), F9 cells (lane B) and NIH/3T3 cells (lane C) and whole cell lysates from mouse thymus cells (lane D) and spleen (lane E). Primary antibody: Anti-Estrogen Receptor β (Ab-1) (485-503) Rabbit pAb (Cat. No. PC168) (1:1000). Detection: chemiluminescence.
References
ReferencesHussien, A., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 1521.
Li, X., et al. 1997. Neuroendocrinology 66, 63.
Pratt, W.B. and Toft, D.O. 1997. Endocrine Rev. 18, 306.
Schumacher, M., et al. 1996. Dev. Neurosci. 18, 6.
Toran-Allerand, C.D. 1996. Dev. Neurosci. 18, 36.
Weigel, N.L. 1996. Biochem. J. 319, 657.
Jones, K.J. 1994. Ann. N.Y. Acad. Sci. 743, 141.
Arnold, A.P. 1990. in Hormones, Brain and Behavior in Vertebrates, ed. Balthazart, J. (Karger, Basel), pp. 82-91.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Positive controlBreast carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (5-10 µg/ml)
Gel Shift (1-5 µl)
Immunoblotting (1 µg/ml)
Paraffin Sections (1:50)
Application CommentsThis antibody will also specifically and efficiently supershift rat estrogen receptor β. Does not cross-react with estrogen receptor α. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (CSSTEDSKNKESSQNLQSQ) corresponding to amino acids 485-503 of rat estrogen receptor β
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Global Trade Item Number
Catalogue Number GTIN
PC168 0