Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

324870 Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-α-ENaC

324870
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
324870-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~97 kDa glycosylated α-ENaC protein.
Catalogue Number324870
Brand Family Calbiochem®
SynonymsAnti-α-ENaC
References
ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
Application CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
324870-100UG 04055977215830

Documentation

Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb Certificates of Analysis

TitleLot Number
324870

References

Reference overview
Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-August-2007 RFH
SynonymsAnti-α-ENaC
ApplicationImmunoblotting (1 µg/ml)
Immunoprecipitation (see comments)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.
BackgroundEpithelial sodium channels are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. ENaC in the kidney, lung, and colon plays a role in trans-epithelial Na+ and fluid balance. ENaC also mediates aldosterone-dependent sodium reabsorption in the distal nephron of the kidney, thus regulating blood pressure. ENaC is believed to be regulated through association with the cystic fibrosis transmembrane conductance regulator (CFTR) chloride ion channel.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein
IsotypeIgG
Specieshuman, rat
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBenos, D.J., and Stanton, B.A. 1999. J. Physiol. London 520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays 21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol. 18, 138.