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07-1820-25UL Anti-EEA1 (rabbit polyclonal)

This Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.

Anti-EEA1 (rabbit polyclonal) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: EEA1, ZFYVE2, MST105, MSTP105 UniProt Number: Q15075
07-1820-25UL
25 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1820-25UL
DescriptionAnti-EEA1 (rabbit polyclonal)
Alternate Names
  • early endosome antigen 1
  • early endosome-associated protein
  • early endosome antigen 1, 162kD
  • Endosome-associated protein p162
  • Zinc finger FYVE domain-containing protein 2
Background InformationEarly endosome antigen 1 (EEA1) is a 170 kDa coiled-coil protein belonging to a group of early endosomal Rab5 effector proteins. EEA1 has a putative role in the tethering/docking of endocytic vesicles prior to their fusion with early endosomes. This protein is characterized as containing a zinc finger-like domain and coiled-coil regions. The zinc finger-like domain, also known as the FYVE finger, is a motif rich in cystine, is a necessary component of EEA1’s ability for endosomal targeting, and is specifically known for its dual interaction with Rab5 GTPase and phosphotidylinositol 3-phosphate (Ptdlns-3-P).
References
Product Information
FormatAffinity Purified
Control
  • BALB 3T3/A31 cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-EEA1 Antibody is validated for use in WB, IC for the detection of EEA1.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blot (SNAP ID) Analysis: 1:250 dilution from a previous lot detected EEA1 on 10 µg of C2C12 cell lysate.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected EEA1 in HeLa, A431, and C2C12 cells.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the C-terminus of human EEA1.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes EEA1.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteDemonstrated to react with Human and Mouse.
Equine and Canine (99% sequence homology). Rat (98% sequence homology). Bovine (97% sequence homology).
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EEA1
  • ZFYVE2
  • MST105
  • MSTP105
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

SUBUNIT STRUCTURE: Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding.

SUBCELLULAR LOCATION: Cytoplasm. Early endosome membrane; Peripheral membrane protein.

DOMAIN: The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate.

MISCELLANEOUS: Antibodies against EEA1 are found in sera from patients with subacute cutaneous lupus erythematosus and other autoimmune diseases.

SEQUENCE SIMILARITIES: Contains 1 C2H2-type zinc finger.

Contains 1 FYVE-type zinc finger.
Molecular Weight~ 170 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in BALB 3T3/A31 cell lysate.

Western Blot Analysis: A 1:1000 dilution of this antibody detected EEA1 on 10 µg of BALB 3T3/A31 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-1820-25UL 04054839350467