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NA49 Anti-E2F1 (Ab-1) Mouse mAb (KH95)

Anti-E2F1 (Ab-1) Mouse mAb (KH95) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the E2F1 protein is found in most cell types.
Catalogue NumberNA49
Brand Family Calbiochem®
References
ReferencesCampanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
Neuman, E., et al. 1996. Gene 173, 163.
Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Positive controlHeLa or Y79 cells
PreservativeNone
Applications
Key Applications Immunoblotting (Western Blotting)
Not Immunofluorescence
Not Immunoprecipitation
Application NotesImmunoblotting (2.5 µg/ml)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Application CommentsThe E2F1 protein is found in most cell types. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant human E2F1
ImmunogenHuman
CloneKH95
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NA49 0

Documentation

Anti-E2F1 (Ab-1) Mouse mAb (KH95) SDS

Title

Safety Data Sheet (SDS) 

Anti-E2F1 (Ab-1) Mouse mAb (KH95) Certificates of Analysis

TitleLot Number
NA49

References

Reference overview
Campanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
Neuman, E., et al. 1996. Gene 173, 163.
Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-September-2007 RFH
ApplicationImmunoblotting (2.5 µg/ml)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
DescriptionPurified mouse monoclonal antibody antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the E2F1 protein.
BackgroundE2F transcription activity is the result of regulation by a family of heterodimers that are encoded by distinct genes. The different members of the E2F family form complexes with the DP1 protein and it is this complex which is responsible for the regulatory activity. The gene for E2F1 was the first of the E2F family to be cloned and was shown to map to chromosome 20q11. The gene contains 7 exons and is regulated at the mRNA levels through changes in transcription. E2F1 has been shown to regulate both G1 and S phase cyclins and in particular there is evidence for a type of feedback regulatory loop between E2F and cyclin D1. Overexpression of E2F1 has been shown to lead to the induction of apoptosis possibly through the inhibition of cyclinD1 dependent kinase activity coupled with the induction of a p16 related transcript. In addition to the transcription level regulation of E2F1, there is also control of E2F1 protein levels by the ubiquitin-proteosome dependent degradation pathway. Ubiquitination is blocked by Rb stabilizing the E2F1 complex formation and directly controlling cell cycle progression.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant human E2F1
CloneKH95
IsotypeIgG₁
Specieshuman
Positive controlHeLa or Y79 cells
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsThe E2F1 protein is found in most cell types. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCampanero, M.R. and Flemington, E.K. 1997. Proc. Natl. Acad. Sci. USA 94, 2221.
DeGregori, J., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 7245.
Fan, J. and Bertino, J.R. 1997. Oncogene 14, 1191.
Khleif, S.N., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 43501.
Neuman, E., et al. 1996. Gene 173, 163.
Slansky, J.E. and Farnham, P.J. 1996. Curr. Top. Microbiol. Immunol. 208, 1.