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AB1537 Sigma-AldrichAnti-Dopamine β Hydroxylase Antibody, CT

This Anti-Dopamine β Hydroxylase Antibody, C-terminus is validated for use in WB, IH for the detection of Dopamine β Hydroxylase.

Anti-Dopamine β Hydroxylase Antibody, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

CoA
References

Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors, Neuronal & Glial Markers Gene Symbol: DBH, DBM, EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase]. UniProt Number: P09172

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, Mk	WB, IHC	Sh	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB1537
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-Dopamine β Hydroxylase Antibody, CT
Alternate Names	DBH

References

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Quality Level	MQ100

Applications
Application	This Anti-Dopamine β Hydroxylase Antibody, C-terminus is validated for use in WB, IH for the detection of Dopamine β Hydroxylase.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunoblotting: 1:250-1:1,000 on human, monkey and bovine adrenal medulla. Immunohistochemistry: 1:250-1:1,000, staining was evaluated in the locus coeruleus of formaldehyde-fixed, cryopreserved human and monkey brain. Showed strong staining on both human and monkey. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide, CPTSQGRSPAGPTVVSI-amide, from C-terminal region of human (DbetaH).
Epitope	C-terminus
Host	Sheep
Specificity	Dopamine Beta Hydroxylase (DbetaH) C-terminal. By immunoblot the antibody specifically reacts with a single (or double) band(s) of M.W. »70-75,000 in samples of SDS-solubilized, DTT-reduced human adrenal medulla subjected to SDS-PAGE.
Species Reactivity	Human Monkey
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000787.3
Entrez Gene Summary	The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide.
Gene Symbol	DBH DBM EC 1.14.17.1 [Contains: Soluble dopamine beta-hydroxylase].
UniProt Number	P09172
UniProt Summary	FUNCTION: SwissProt: P09172 # Conversion of dopamine to noradrenaline. COFACTOR: Binds 1 PQQ per subunit. & Binds 2 copper ions per subunit. SIZE: 617 amino acids; 69065 Da SUBUNIT: Homotetramer composed of two non-covalently bound disulfide-linked dimers. SUBCELLULAR LOCATION: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. & Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single- pass type II membrane protein (Potential). DISEASE: SwissProt: P09172 # Defects in DBH are the cause of DBH deficiency [MIM:223360]; also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction. SIMILARITY: SwissProt: P09172 ## Belongs to the copper type II ascorbate-dependent monooxygenase family. & Contains 1 DOMON domain.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze-thaw cycles.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
AB1537	04053252373886

Documentation

Anti-Dopamine β Hydroxylase Antibody, CT Certificates of Analysis

Title	Lot Number
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2398115	2398115
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE -2604221	2604221
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 3849144	3849144
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 3981688	3981688
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 4001563	4001563
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 4014590	4014590
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 4080455	4080455
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL - 4107221	4107221
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL -2679444	2679444
SHEEP ANTI-DOPAMINE BETA HYDROXYLASE C-TERMINAL AFFINITY PURIFIED POLYCLONAL ANTIBODY	2876888

References

Reference overview	Application	Species	Pub Med ID
Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells Yan, Yiping, et al. Stem Cells, 23:781-790 () 2005	Immunocytochemistry	Human	15917474
Antibodies raised against different oligopeptide segments of human dopamine-beta-hydroxylase. Nagatsu, I, et al. Neurosci. Lett., 120: 141-5 (1990) 1990 Show Abstract We raised antibodies against 3 oligopeptide segments of human dopamine-beta-hydroxylase (hDBH) corresponding to the N-terminal (hDBH-N), the intermediate (hDBH-I), and the C-terminal (hDBH-C) amino acid sequences (residues 26-43, 452-468, and 582-598), respectively. We characterized the antibodies in terms of specificity by means of Western blotting and immunohistochemistry. Anti-hDBH-N antiserum recognized DBH in the brain (noradrenergic neurons in the pons and medulla oblongata) and adrenal medulla, not only of human but also of mouse, rat and house shrew. In contrast, anti-hDBH-C antiserum recognized only human DBH. These observations suggest that the antibody raised against the hDBH-C terminal peptide may specifically recognize only human DBH.			2293101

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies

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