Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IF14 Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10)

Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
IF14
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~45 kDa cytokeratin 18 protein in a majority of adenocarcinoma and aductal carcinomas, but not squamos cell carcinomas.
Catalogue NumberIF14
Brand Family Calbiochem®
Application Data
Detection of human cytokeratin 18 by immunohistochemistry. Sample: Human breast cancer tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: fluorescence.

Detection of human Cytokeratin 18 by immunoblotting. Sample: Human cytokeratin 18. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesCarley, W.W., et al. 1996. Microcirculation 3, 359.
Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
Rafiee, P., et al. 1992. 7, 123.
Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
Chung-Welch, N., et al. 1989. Differentiation 42, 44.
Jahn, L., et al. 1987. Differentiation 36, 234.
Moll, R., et al. 1983. Differentiation 23, 256.
Geisler, N., et al. 1982. Cell 30, 277.
Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
Moll, R., et al. 1982. Cell 31, 11.
Osborn, M. and Weber, K., 1982. Cell 31, 303.
Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.
Product Information
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Negative controlArterial blood vessels or HUVECs
Positive controlHeLa cells, mesothelial cells, or skin or hepatocellular carcinoma tissue
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (1-2 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunofluorescence (see comments)
Paraffin Sections (1-2 µg/ml, pressure cooker pre-treatment required)
Application CommentsThis antibody reacts with a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia. It has been reported that tissues from the gastrointestinal tract are positive for both CK8 and CK18 but do not contain CK14. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenhuman PMC42 breast cancer cells
ImmunogenHuman
CloneDC10
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IF14 0

Documentation

Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) SDS

Title

Safety Data Sheet (SDS) 

Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) Certificates of Analysis

TitleLot Number
IF14

References

Reference overview
Carley, W.W., et al. 1996. Microcirculation 3, 359.
Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
Rafiee, P., et al. 1992. 7, 123.
Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
Chung-Welch, N., et al. 1989. Differentiation 42, 44.
Jahn, L., et al. 1987. Differentiation 36, 234.
Moll, R., et al. 1983. Differentiation 23, 256.
Geisler, N., et al. 1982. Cell 30, 277.
Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
Moll, R., et al. 1982. Cell 31, 11.
Osborn, M. and Weber, K., 1982. Cell 31, 303.
Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-August-2007 RFH
ApplicationFrozen Sections (1-2 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunofluorescence (see comments)
Paraffin Sections (1-2 µg/ml, pressure cooker pre-treatment required)
Application Data
Detection of human cytokeratin 18 by immunohistochemistry. Sample: Human breast cancer tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: fluorescence.

Detection of human Cytokeratin 18 by immunoblotting. Sample: Human cytokeratin 18. Primary antibody: Anti-Cytokeratin 18 (Ab-2) Mouse mAb (DC10) (Cat. No. IF14) (1 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with p3 X63.Ag8.653 mouse myeloma cells. Recognizes the ~45 kDa cytokeratin 18 protein.
BackgroundIntermediate (10 nm) filaments (IFs) are present in, and provide information concerning the origin of, most vertebrate cells. At present, five major cell types can be distinguished by their intermediate filament components. These include epithelial cells (cytokeratins), neurons (neurofilaments), glial cells (GFA), skeletal, visceral and certain vascular smooth muscle cells (desmin) and various nonepithelial cells, including cells of mesenchymal origin (vimentin). Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of ~40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Cytokeratin 18 (CK18) is an acidic keratin which is found primarily in non-squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. CK18 exists in combination with CK8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only cytokeratins 8 and 18.
HostMouse
Immunogen speciesHuman
Immunogenhuman PMC42 breast cancer cells
CloneDC10
IsotypeIgG₁
Specieshuman
Positive controlHeLa cells, mesothelial cells, or skin or hepatocellular carcinoma tissue
Negative controlArterial blood vessels or HUVECs
FormLiquid
FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody reacts with a variety of simple epithelia including glandular epithelium but not stratified squamous epithelia. It has been reported that tissues from the gastrointestinal tract are positive for both CK8 and CK18 but do not contain CK14. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFor long-term storage, aliquot and freeze (-20°C). Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesCarley, W.W., et al. 1996. Microcirculation 3, 359.
Herzig, K.H., et al. 1994. Gastroenterology 106 1326.
Rafiee, P., et al. 1992. 7, 123.
Dodge, A.B., et al. 1991. Comp. Biochem. Physiol. 98, 461.
Patton, W.F., et al. 1990. J. Cell. Physiol. 143, 140.
Stosiek, P., et al. 1990. Acta Histochem. 89, 61.
Chung-Welch, N., et al. 1989. Differentiation 42, 44.
Jahn, L., et al. 1987. Differentiation 36, 234.
Moll, R., et al. 1983. Differentiation 23, 256.
Geisler, N., et al. 1982. Cell 30, 277.
Lazarides, E., 1982. Ann. Rev. Biochem. 51, 219.
Moll, R., et al. 1982. Cell 31, 11.
Osborn, M. and Weber, K., 1982. Cell 31, 303.
Fuchs, E.V. and Coppock, S.M. 1981. Cell 27, 75.