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MABF2816-100UG Anti-Cortical stromal cells Antibody, clone ER-TR4

Anti-Cortical stromal cells, clone ER-TR4, Cat. No. MABF2816, is a rat monoclonal antibody that detects mouse thymus cortical epithelial cells and is tested for use in Immunofluorescence and Immunohistochemistry.

MABF2816-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2816-100UG
DescriptionAnti-Cortical stromal cells Antibody, clone ER-TR4
Alternate Names
  • cTEC
Background InformationThe thymus is considered as the primary lymphoid organ that is responsible for the generation and maturation of T cells. Thymic epithelial cells (TECs) account for the majority of thymic stromal components. These cells can be divided into cortical and medullary thymic epithelial cells based on their localization within the thymus. TECs have dual germ layer origins, with cortical cells arising from ectoderm and medullary cells arising from the endoderm. In an embryonic thymus, cortical cells are the dominant population, while medullary cells account for most of the TECs in the adult thymus. Cortical thymic epithelial cells are essential for the positive selection of T cells, whereas the medullary thymic epithelial cells (mTECS) play a role in inducing negative selection of highly reactive T cells that are required for establishing central self-tolerance. mTECs participate in self-tolerance by eliminating self-reactive T cells. Cortical cells that support positive selection also support post-selection maturation of single-positive thymocytes. They provide cytokines, such as IL-7 and SCF, to promote early thymic progenitors (ETP) cells and Delta-like ligand 4 (DLL4)-mediated Notch signaling to induce the differentiation of ETPs towards the T lineage. DLL4 is reported to be expressed at high levels in cortical cells, but not in medullary cells. Clone ER-TR4 reacts with epithelial-reticular cells in the cortex of murine thymus. (Ref.: Wang, HX., et al. (2020). Front. Immunol. 10; 3099; Van Vliet, E., et al. (1984). Eur. J. Immunol. 14(6); 524-529; Cordier, AC., et al. (1980). Am. J Anat. 157(3); 227-263).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Cortical stromal cells, clone ER-TR4, Cat. No. MABF2816, is a rat monoclonal antibody that detects mouse thymus cortical epithelial cells and is tested for use in Immunofluorescence and Immunohistochemistry.
Key Applications
  • Immunohistochemistry
  • Immunofluorescence
Application NotesTested applications

Immunofluorescence Analysis: A representative lot detected Cortical stromal cells in Immunofluorescence applications (Tanaka, Y., et al., (2007). Blood. 110(1):107-15).

Immunohistochemistry Applications: A representative lot detected Cortical stromal cells in Immunohistochemistry applications (Van Vliet, E., et al., (1984). Eur J Immunol. 14(6):524-9; Scheijen, B., et al., (2004). J Biol Chem. 279(11):10476-83; Dooley, J., et al., (2006). J Immunol. 176(11):6484-90; Tanaka, Y., et al., (2007). Blood. 110(1):107-15; Moretti, F.A., et al., (2018). Elife. 7:e35816; Hollander, G.A., et al. (1995). Nature. 373(6512):350-3).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenThymic stromal cells isolated from C3H mice.
EpitopeUnknown
CloneER-TR4
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone ER-TR4 is a rat monoclonal antibody that detects cortical stromal cells.
IsotypeIgMκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodLigatrap
UniProt Number
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in mouse thymus tissue sections.

Immunohistochemistry Applications: A 1:50 dilution of this antibody detected Cortical stromal cells in mouse thymus tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2816-100UG 04065268979051