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ABS1680 Anti-Chromatin-Modifying Protein 2a

Anti-Chromatin-Modifying Protein 2a, Cat. No. ABS1680, is a rabbit polyclonal antibody that targets Charged multivesicular body protein 2a and has been tesed for use in Western Blotting.

Anti-Chromatin-Modifying Protein 2a MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CHMP2A, BC2, CHMP2 UniProt Number: O43633
ABS1680
50 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1680
DescriptionAnti-Chromatin-Modifying Protein 2a
Alternate Names
  • Charged multivesicular body protein 2a
  • CHMP2a
  • Putative breast adenocarcinoma marker BC-2
  • Vacuolar protein sorting-associated protein 2-1
  • Vps2-1
  • hVps2-1
Background InformationCharged multivesicular body protein 2a (UniProt: O43633; also known as Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1) is encoded by the CHMP2A (also known as BC2, CHMP2) gene (Gene ID: 27243) in human. CHMP2a is a core component of the endosomal sorting required for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It localizes to the mid-body of dividing cells. CHMP2a consists of an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. Cells lacking CHMP2A exhibit mitotic defects. They form transient monopolar spindles and fail to segregate chromosomes normally and also fail to complete abscission. Cell death is also reported to be higher in cells depleted of CHMP2A, CHMP4B, and VPS4B.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Chromatin-Modifying Protein 2a, Cat. No. ABS1680, is a rabbit polyclonal antibody that targets Charged multivesicular body protein 2a and has been tesed for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Chromatin-Modifying Protein 2a in Western Blotting applications (Morita, E., et. al. (2010). Proc Natl Acad Sci USA. 107(29):12889-94; Bajorek, M., et. al. (2009). Nat Struct Mol Biol. 16(7):754-62).
Biological Information
ImmunogenGST-tagged full-length human recombinant Chromatin-modifying protein 2a.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specifically detects human Chromatin-modifying protein 2a.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHMP2A
  • BC2
  • CHMP2
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~32 kDa observed; 25.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Chromatin-Modifying Protein 2a in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS1680 04054839201332