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AB144 Anti-Choline Acetyltransferase Antibody

AB144
50 µL  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Gp, M, RIHC, WBGtSerumPolyclonal Antibody
Description
Catalogue NumberAB144
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Choline Acetyltransferase Antibody
Alternate Names
  • ChAT
Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
Product Information
FormatSerum
Control
  • Rat brain tissue
PresentationGoat antisera, liquid, no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Choline Acetyltransferase Antibody is an antibody against Choline Acetyltransferase for use in IH & WB.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunoblot: 1:1,000-1:5,000

Immunohistochemistry: 1:500-1:1,000. See page two of this data sheet.

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman placental enzyme.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostGoat
SpecificityRecognizes cholinergic neurons in brain and central nervous systems.
Species Reactivity
  • Guinea Pig
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein.
Gene Symbol
  • CHAT
  • ChAT
  • CHOACTase
  • CMS1A
  • CMS1A2
Purification MethodUnpurified
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
SIZE: 748 amino acids; 82568 Da
DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
Molecular Weight70 kDa
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Packaging Information
Material Size50 µL
Global Trade Item Number
Catalogue Number GTIN
AB144 04053252401879