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MABT1323-100UL Anti-Cdh1 Antibody, clone AR38.2

Anti-Cdh1, clone AR38, Cat. No. MABT1323, is a mouse monoclonal antibody that detects Cdh1/FZR1 and has been tested for use in Western Blotting.

Anti-Cdh1 Antibody, clone AR38.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Fzr1, Fye, Fzr UniProt Number: Q9R1K5
MABT1323-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1323-100UL
DescriptionAnti-Cdh1 Antibody, clone AR38.2
Alternate Names
  • Fizzy-related protein homolog
  • Fzr
  • Cdh1/Hct1 homolog
Background InformationFizzy-related protein homolog (UniProt: Q9R1K5; also known as Fzr, Cdh1/Hct1 homolog) is encoded by the Fzr1 (also known as Fyr, Fzr) gene (Gene ID: 56371) in murine species. Cdh1 is a substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex that associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition Cdh1 is phosphorylated, leading to its dissociation from the APC/C. It has been shown that Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while CDC14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to ubiquitination and degradation of Polo-like kinase 1 (Plk1) and prevents entry into mitosis. Following DNA repair, Cdk1 and Plk1 are reactivated and cell cycle progresses from G2 into mitosis phase.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Cdh1, clone AR38, Cat. No. MABT1323, is a mouse monoclonal antibody that detects Cdh1/FZR1 and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Cdh1 in Western Blotting applications (Garzon, J., et. al. (2017). Oncogene. 36(42):5808-5818).
Biological Information
ImmunogenHis-tagged full-length recombinant mouse Fizzy-related protein homolog (Cdh1).
CloneAR38.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone AR38.2 detects Cdh1 (Fizzy-related protein homolog) in human and mouse cells.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Fzr1
  • Fye
  • Fzr
Purification MethodProtein G purified
UniProt Number
Molecular Weight~54 kDa observed; 54.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cdh1 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1323-100UL 04054839589652