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MAB131872 Anti-Cas9 Antibody, clone 6F7

Anti-Cas9, clone 6F7, Cat. No. MAB131872, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunocytochemistry and Western Blottin

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Anti-Cas9 Antibody, clone 6F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cas9 UniProt Number: J7RUA5
MAB131872
100 μg  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB131872
DescriptionAnti-Cas9 Antibody, clone 6F7
Alternate Names
  • CRISPR-associated endonuclease Cas9
  • SaCas9
Background InformationCRISPR-associated endonuclease Cas9 (UniProt: J7RUA5; also known as SaCas9, Cas9) is encoded by the Cas9 gene in Streptococcus aureus. Cas9 belongs to subtype II-A subfamily with one HNH Cas9-type domain. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. Cas9 has a bilobed architecture with a recognition lobe (residues 41-425) and a discontinuous nuclease lobe (NUC, residues 1-40 and 453-1053); the crRNA-target DNA lies in a channel between the 2 lobes. The NUC lobe has 2 endonuclease domains. The discontinuous RuvC-like domain in NUC cleaves the target DNA non-complementary to crRNA while the HNH nuclease domain cleaves the target DNA complementary to crRNA. Cas9 serves as a RNA-guided DNA endonuclease that cleaves double-stranded DNA targets with a protospacer adjacent motif (PAM) and complementarity to the guide RNA. The homologous region of the Streptococcus aureus is not closely related in amino acid sequence in S. aureus, hence this antibody does not recognize that protein.
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 0.03% sodium azide and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Cas9, clone 6F7, Cat. No. MAB131872, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9 and has been tested in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Cas9 in HEK293 cells which overexpress fusion protein containing GFP and C-terminus of Cas9 from S. aureus (Courtesy of Dr Gerry Shaw).

Western Blotting Analysis: A representative lot detected Cas9 in HEK293 overexpressing fusion protein containing GFP and C-terminus of Cas9 from S. aureus (Courtesy of Dr Gerry Shaw).
Biological Information
ImmunogenA recombinant fragement corresponding to 251 amino acids from the C-terminal region of Staphylococcus aureus. Expressed in and purified from E. coli.
EpitopeC-terminus
Clone6F7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6F7 specifically detects Cas9 in Staphylococcus aureus. It does not recognize Cas9 in Streptococcus pyogenes.
IsotypeIgG1κ
Species Reactivity
  • Mouse
Species Reactivity NoteStaphylococcus aureus.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cas9
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~53 kDa observed (truncated form). Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysates overexpressing a fusion protein containing GFP and C-terminus of Cas9 from Staphylococcus aureus.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cas9 in 10 µg of HEK293 cell lysates overexpressing a fusion protein containing GFP and c-terminus of Cas9 from Staphylococcus aureus.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MAB131872 04054839178931