Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

208729 Anti-Calpain-2, Domains III/IV Mouse mAb (107-82)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-m-Calpain, Domains III/IV

208729
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.



We apologize for the inconvenience, but we do not currently have an alternative product.






Recognizes the ~80 kDa subunit of calpain-2.
Catalogue Number208729
Brand Family Calbiochem®
SynonymsAnti-m-Calpain, Domains III/IV
References
ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
Product Information
FormLiquid
FormulationAscites diluted in PBS.
Positive controlHuman platelets or erythrocytes, bovine platelets, heart, or skeletal muscle, or rat myoblasts, kidney, liver, or spleen
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunofluorescence
Application NotesImmunoblotting (1:500)
Immunocytochemistry (1:50)
Application CommentsDoes not cross-react with calpain-1, n-calpain, calmodulin, or calpastatin. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified calpain-2 from bovine skeletal muscle
ImmunogenBovine
EpitopeWithin amino acids 502-699 of calpain 2 domains III/IV
Clone107-82
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
208729 0

Documentation

Anti-Calpain-2, Domains III/IV Mouse mAb (107-82) Certificates of Analysis

TitleLot Number
208729

References

Reference overview
Cong, J., et al. 1993. J. Biol. Chem. 268, 25740.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision24-July-2007 RFH
SynonymsAnti-m-Calpain, Domains III/IV
ApplicationImmunoblotting (1:500)
Immunocytochemistry (1:50)
DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x3Ag8.653 mouse myeloma cells. Recognizes the ~80 kDa subunit of calpain-2.
BackgroundCalpains are calcium-dependent thiol-proteases that proteolyze a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain-1 (µ-form) and calpain-2 (m-form), differ in their calcium requirement for activation. They are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme, whereas the 30 kDa unit is the regulatory subunit and is common to both µ- and m-isozymes. The 80 kDa subunit consists of four domains (I-IV) and the 30 kDa unit has 2 domains (V and VI). Domain I is partially removed during autolysis. Domain II is the protease domain.
HostMouse
Immunogen speciesBovine
Immunogenpurified calpain-2 from bovine skeletal muscle
EpitopeWithin amino acids 502-699 of calpain 2 domains III/IV
Clone107-82
IsotypeIgG₁
Speciesbovine, human, porcine, rat
Positive controlHuman platelets or erythrocytes, bovine platelets, heart, or skeletal muscle, or rat myoblasts, kidney, liver, or spleen
FormLiquid
FormulationAscites diluted in PBS.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with calpain-1, n-calpain, calmodulin, or calpastatin. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.