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MABC1593-25UG Anti-CRBN Antibody, clone 4D6

Anti-CRBN, clone 4D6 , Cat. No. MABC1593, is a mouse monoclonal antibody that detects Cereblon and has been tested for use in Immunoprecipitation and Western Blotting.

MABC1593-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABC1593-25UG
DescriptionAnti-CRBN Antibody, clone 4D6
Alternate Names
  • Protein Cereblon
  • CRBN
Background InformationProtein cereblon (UniProt: Q96SW2) is encoded by the CRBN (also known s AD-006) gene (Gene ID: 51185) in human. Cereblon is a widely expressed peripheral membrane protein that serves as a substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. The cullin-4 (CULT4) protein provides a scaffold for assembling RING box-domain protein (RBX1) and the adaptor protein damaged DNA-binding protein 1 (DDB1) to form a CRL4 E3 complex, where RBX1 serves as the docking site for the activated E2 protein, and DDB1 recruits substrate-specificity receptors or DCAFs (DDB1-cullin 4-associated factors) to form the substrate-presenting side of the CRL4 complex. Cereblon has been recognized as the primary teratogenic target of thalidomide and its analogs. These drugs exert their antiproliferative effects on multiple myeloma cells and immunomodulatory effects on T cells by directly binding cereblon and promoting recruitment of the hematopoietic zinc-finger transcription factors Ikaros (IKZF1) and Aiolos (IKZF3), leading to their ubiquitination and proteasomal degradation. Human cereblon is a 442-amino acid protein composed of a Lon protease-like scaffold (aa 80-317) with a helical insertion that mediates DDB1 binding and a thalidomide-binding domain (aa 339-442).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-CRBN, clone 4D6 , Cat. No. MABC1593, is a mouse monoclonal antibody that detects Cereblon and has been tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: 20 ug from a representative lot detected CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CRBN in MOLT4 wild type cell lysates (Courtesy of Stefan Schuchner, Ph.D. and Egon Ogris, M.D., Medical University of Vienna Austria).
Biological Information
ImmunogenHis-tagged recombinant protein fragment corresponding to the N-terminal region of human Cereblon.
Clone4D6 (-3H10-D6)
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 4D6 (-3H10-D6) is a mouse monoclonal antibody that detects human Cereblon. It targets an epitope within the N-terminal domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CRBN
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in MOLT-4 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Cereblon.(CRBN) in MOLT-4 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1593-25UG 04061841039150