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MABE455 Anti-CHD-4 Antibody, clone 3F2/4

Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.

Anti-CHD-4 Antibody, clone 3F2/4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: CHD4 UniProt Number: Q14839
MABE455
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MChIP, WB, IHC, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE455
DescriptionAnti-CHD-4 Antibody, clone 3F2/4
Alternate Names
  • Chromodomain-helicase-DNA-binding protein 4
  • CHD-4
  • ATP-dependent helicase CHD4
  • Mi-2 autoantigen 218 kDa protein
  • Mi2-beta
Background InformationCHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
References
Product Information
FormatPurified
Control
  • HeLa cell lysate
PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Western Blotting
  • Immunohistochemistry
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
Biological Information
ImmunogenLinear peptide corresponding to human CHD-4.
Clone3F2/4
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CHD4
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~220 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE455 04053252920936

Documentation

Anti-CHD-4 Antibody, clone 3F2/4 SDS

Title

Safety Data Sheet (SDS) 

Anti-CHD-4 Antibody, clone 3F2/4 Certificates of Analysis

TitleLot Number
Anti-CHD-4, clone 3F2/4 - 3252104 3252104
Anti-CHD-4, clone 3F2/4 - 3530664 3530664
Anti-CHD-4, clone 3F2/4 - 3532474 3532474
Anti-CHD-4, clone 3F2/4 - 3700366 3700366
Anti-CHD-4, clone 3F2/4 - 3822329 3822329
Anti-CHD-4, clone 3F2/4 - 4069762 4069762
Anti-CHD-4, clone 3F2/4 - 4093866 4093866
Anti-CHD-4, clone 3F2/4 - Q2239051 Q2239051
Anti-CHD-4, clone 3F2/4 Monoclonal Antibody 2972041