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ABE1860 Anti-CDK13

Anti-CDK13, Cat. No. ABE1860, is a highly specific rabbit polyclonal antibody that targets Cyclin-dependent kinase 13 and has been tested in Western Blotting.

Anti-CDK13 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: CDK13, CDC2L, CDC2L5, CHED, KIAA1791 UniProt Number: Q14004
ABE1860
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE1860
DescriptionAnti-CDK13
Alternate Names
  • Cyclin-dependent kinase 13
  • CDC2-related protein kinase 5
  • Cell division cycle 2-like protein kinase 5
  • Cell division protein kinase 13
  • hCDK13
  • Cholinesterase-related cell division controller
Background InformationCyclin-dependent kinase 13 (UniProt: Q14004: also known as Cdk13, EC:2.7.11.22, EC:2.7.11.23, CDC2-related protein kinase 5, Cell division cycle 2-like protein kinase 5, Cell division protein kinase 13, hCDK13, Cholinesterase-related cell division controller) is encoded by the CDK13 (also known as CDC2L, CDC2L5, CHED, KIAA1791) gene (Gene ID:8621) in human. CDK13 is a member of the cyclin-dependent serine/threonine protein kinase family. Members of this family are known to play a vital role as master switches in cell cycle control. Human CDK 13 shares 43% sequence identity with CDK12. CDK13 is expressed in fetal brain, liver, muscle, in adult brain and in neuroblastoma and glioblastoma tumors. It contains expanded regions of serine-arginine (SR) motifs in its N-terminal regions, which links it to the SR protein family involved in RNA processing and pre-mRNA splicing. CDK13 contains a C-terminal extension helix that is composed of a polybasic cluster and a DCHEL motif that interacts with the bound ATP. CDK13 displays C-terminal repeat domain (CTD) kinase activity and is required for RNA splicing. It hyperphosphorylates the C-terminal heptapeptide repeat domain of the largest RNA polymerase II subunit RPB1 and acts a key regulator of transcription elongation. Phosphorylation of the three serine residues (Ser 2, 5, and 7) within the CTD is linked to the phases of RNAPII-mediated transcription. Phosphorylation of Ser7 (pSer7) starts the transcription cycle that primes the CTD for further modifications. (Ref.:Greifenberg, AK et al. (2016). Cell Reports 14(2): 320-331).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CDK13, Cat. No. ABE1860, is a highly specific rabbit polyclonal antibody that targets Cyclin-dependent kinase 13 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected CDK13 in HeLa cell lysate (Bartkowiak, B., et. al. (2010). Genes Dev. 24(20):2303-16).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HEK293 whole cell extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in HeLa nuclear extract. (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).

Western Blotting Analysis: 1:1,000 dilution from a representative lot detected CDK13 in OVCA5 and HEK293 (Courtesy of Dr. A. Greenleaf, Duke University Medical Center).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the N-terminal half of human Cyclin-dependent kinase 13 (CDk13).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specifically detects CDK13 in HEK293 cells.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CDK13
  • CDC2L
  • CDC2L5
  • CHED
  • KIAA1791
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~170 kDa observed; 164.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 whole cell extract.

Western Blotting Analysis: 1:500 dilution of this antibody detected CDK13 in 20 µL of HEK293 whole cell extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1860 04054839169649