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217952 Anti-CD95L Mouse mAb (Alf-2.1a) Fluorescein Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Fas Ligand, FITC

217952
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes membrane bound and soluble human Fas ligand in KFL9 cells.
Note: 1 T = 1 test.
Catalogue Number217952
Brand Family Calbiochem®
SynonymsAnti-Fas Ligand, FITC
References
ReferencesSuda, T., et al. 1997. J. Exp. Med. 186, 2045.
Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 5% glycerol, 0.2% BSA, pH 7.5.
Negative controlHPB-MLT cells
Positive controlKFL9 cells, full length CD95L transfectants
Preservative≤0.1% sodium azide
Applications
Key Applications Flow Cytometry
Neutralization Studies
Application NotesFlow Cytometry (1:50, 80 µl/test)
Neutralization Studies (see comments)
Application CommentsThis antibody recognizes both membrane-bound and soluble Fas ligand and can block Fas ligand activity. FACS was performed on 5x105 KFL9 cells and analyzed by flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogensoluble human Fas ligand
ImmunogenHuman
CloneAlf-2.1a
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
217952 0

Documentation

Anti-CD95L Mouse mAb (Alf-2.1a) Fluorescein Conjugate Certificates of Analysis

TitleLot Number
217952

References

Reference overview
Suda, T., et al. 1997. J. Exp. Med. 186, 2045.
Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
SynonymsAnti-Fas Ligand, FITC
ApplicationFlow Cytometry (1:50, 80 µl/test)
Neutralization Studies (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~40 kDa Fas ligand (CD95L) protein.
BackgroundHuman Fas ligand (CD95L) is a ~40 kDa type II membrane protein that induces apoptosis in cells expressing Fas (CD95). Fas ligand is one of the major effectors of CD8+ cytotoxic T lymphocytes and NK activity. Loss of Fas ligand leads to lymphoproliferative and autoimmune disease.
HostMouse
Immunogen speciesHuman
Immunogensoluble human Fas ligand
CloneAlf-2.1a
IsotypeIgG₁
Specieshuman
Positive controlKFL9 cells, full length CD95L transfectants
Negative controlHPB-MLT cells
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 5% glycerol, 0.2% BSA, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody recognizes both membrane-bound and soluble Fas ligand and can block Fas ligand activity. FACS was performed on 5x105 KFL9 cells and analyzed by flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
Storage Protect from light
+2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesSuda, T., et al. 1997. J. Exp. Med. 186, 2045.
Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.