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217952 Anti-CD95L Mouse mAb (Alf-2.1a) Fluorescein Conjugate

217952
  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes membrane bound and soluble human Fas ligand in KFL9 cells.
Note: 1 T = 1 test.
Catalogue Number217952
Brand Family Calbiochem®
SynonymsAnti-Fas Ligand, FITC
References
ReferencesSuda, T., et al. 1997. J. Exp. Med. 186, 2045.
Takahashi, T., et al. 1994. Intl. Immunol. 6, 1567.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, 5% glycerol, 0.2% BSA, pH 7.5.
Negative controlHPB-MLT cells
Positive controlKFL9 cells, full length CD95L transfectants
Preservative≤0.1% sodium azide
Applications
Key Applications Flow Cytometry
Neutralization Studies
Application NotesFlow Cytometry (1:50, 80 µl/test)
Neutralization Studies (see comments)
Application CommentsThis antibody recognizes both membrane-bound and soluble Fas ligand and can block Fas ligand activity. FACS was performed on 5x105 KFL9 cells and analyzed by flow cytometry. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogensoluble human Fas ligand
ImmunogenHuman
CloneAlf-2.1a
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Global Trade Item Number
Catalogue Number GTIN
217952 0