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MABT914 Anti-CD9 Antibody, clone Z9.1

This mouse monoclonal Anti-CD9 antibody, clone Z9.1, Cat. No. MABT914, is validated for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of CD9.

Anti-CD9 Antibody, clone Z9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: CD9, MIC3, TSPAN29, GIG2 UniProt Number: P21926
MABT914
50 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, FC, WB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT914
DescriptionAnti-CD9 Antibody, clone Z9.1
Alternate Names
  • 5H9 antigen
  • Cell growth-inhibiting gene 2 protein
  • Leukocyte antigen MIC3
  • Motility-related protein
  • MRP-1
  • Tetraspanin-29
  • Tspan-29
  • p24)
Background InformationCD9 antigen (UniProt: P21926; also known as 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24) is encoded by the CD9 (also known as MIC3, TSPAN29; IG2) gene (Gene ID: 928) in human. CD9 is a multi-pass membrane glycoprotein of the tetraspanin (TM4SF) family. It is expressed in a variety of hematopoietic and epithelial cells. It displays strong expression in platelets where it mediates platelet activation and aggregation upon binding to monoclonal antibodies. CD9 induces cell-cell adhesion, platelet aggregation, and B cell development and is shown to associate with CD63, CD81, CD82, and CD36 and to bind to beta-1 integrins. CD9 activation of platelets is reported to be mediated by the Fc y RII (CD32) molecule. Downregulation of CD9 correlates with tumor progression in some forms of cancer. It can hamper metastasis formation by inhibiting integrin-mediated motility. Lower expression of CD9 are associated with weaker matrix adhesion and diffuse tumor cell growth in vitro.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis mouse monoclonal Anti-CD9 antibody, clone Z9.1, Cat. No. MABT914, is validated for use in ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of CD9.
Key Applications
  • ELISA
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human tonsil, human breast cancer, and human skin tissues.

Flow Cytometry Analysis: A 1:1,000 dilution from a representative lot detected CD9 in human PBMCs.
human tonsil, human breast cancer, human liver cancer, human skin
Biological Information
ImmunogenIntact LoVo colorectal carcinoma cells.
Epitopeextracellular domain
CloneZ9.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone Z9.1 detects CD9 in its native, non-reduced form, in CD-9 positive cells amongst human PBMCs.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD9
  • MIC3
  • TSPAN29
  • GIG2
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.42 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human human liver cancer tissue.

Immunohistochemistry Analysis: A 1:1000 dilution of this antibody detected CD9 in human liver cancer tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size50 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT914 04054839153198