Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

217580 Anti-CD8 Mouse mAb (UCHT4)

Anti-CD8 Mouse mAb (UCHT4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
217580
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~33 kDa CD8 protein on human T lymphocytes.
Catalogue Number217580
Brand Family Calbiochem®
References
ReferencesFischer, A., et al. 1983. Immunology 48, 177.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 100 mM potassium chloride, 50 mM sodium phosphate, pH 7.5.
Preservative0.5 mg/ml gentamycin sulfate
Applications
Key Applications Flow Cytometry
Frozen Sections
Immunoprecipitation
Application NotesFlow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
Application CommentsFACS was performed on 5x105 ficoll prepared human peripheral white blood cells and analyzed by flow cytometry. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenhuman thymocytes or Sezary T cells
ImmunogenHuman
CloneUCHT4
HostMouse
Purity>95% immunoglobulin by SDS-PAGE
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsIn cases where freezing is desired, avoid freeze/thaw cycles.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
217580 0

Documentation

Anti-CD8 Mouse mAb (UCHT4) Certificates of Analysis

TitleLot Number
217580

References

Reference overview
Fischer, A., et al. 1983. Immunology 48, 177.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
ApplicationFlow Cytometry (5 µg/ml)
Frozen Sections (see comments)
Immunoprecipitation (see comments)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~33 kDa CD8 α-chain protein.
BackgroundHuman CD8 is a heterodimeric protein consisting of an α and β chain, each about 33 kDa. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the α2 and α3 domain of MHC Class molecules.
HostMouse
Immunogen speciesHuman
Immunogenhuman thymocytes or Sezary T cells
CloneUCHT4
IsotypeIgG2a
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 100 mM potassium chloride, 50 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Purity>95% immunoglobulin by SDS-PAGE
Preservative0.5 mg/ml gentamycin sulfate
CommentsFACS was performed on 5x105 ficoll prepared human peripheral white blood cells and analyzed by flow cytometry. Optimal binding concentration tested was 5 µg/ml when used with secondary reagent goat anti-mouse IgG, FITC conjugated. This antibody has also been reported to work for frozen sections and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsIn cases where freezing is desired, avoid freeze/thaw cycles.
Toxicity Standard Handling
ReferencesFischer, A., et al. 1983. Immunology 48, 177.