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MABF1565 Anti-CD4 (Mouse), APC, clone RM4-5 Antibody

This Anti-CD4 (Mouse), APC, clone RM4-5 Antibody is validated for use in Flow Cytometry for the detection of CD4.

Anti-CD4 (Mouse), APC, clone RM4-5 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: Cd4 UniProt Number: P06332
MABF1565
25 μg  
Purchase on Sigma-Aldrich

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFCRAPCMonoclonal Antibody
Description
Catalogue NumberMABF1565
DescriptionAnti-CD4 (Mouse), APC, clone RM4-5 Antibody
Alternate Names
  • T-cell surface glycoprotein CD4
  • T-cell differentiation antigen L3T4
  • T-cell surface antigen T4/Leu-3
  • CD4
  • CD4
Background InformationThe RM4-5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The RM4-5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the RM4-5 antibody and an alternative antibody, Anti-Mouse CD4 clone GK1.5, will “compete” for binding, i.e. RM4-5 antibody is able to block GK1.5 antibody binding to cells. In contrast, RM4-5 antibody does not block the binding of Anti-Mouse CD4 clone RM4-4 to cells.
Product Information
FormatAPC
PresentationPurified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD4 (Mouse), APC, clone RM4-5 Antibody is validated for use in Flow Cytometry for the detection of CD4.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRM4-5
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cd4
Purification MethodPurified by size-exclusion chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD4 in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
MABF1565 04055977292718