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MAB3706 Anti-CD4 Antibody, intracellular, clone 024-10D6.B3

Detect CD4 using this Anti-CD4 Antibody, intracellular, clone 024-10D6.B3 validated for use in ELISA, EMSA, FUNC & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD4, CD4mut UniProt Number: P01730
MAB3706
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HELISA, EMSA, FUNC, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB3706
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CD4 Antibody, intracellular, clone 024-10D6.B3
References
Product Information
FormatPurified
PresentationPurified immunoglobulin. Liquid in 0.02 M phosphate buffer, 0.25M NaCl, with 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect CD4 using this Anti-CD4 Antibody, intracellular, clone 024-10D6.B3 validated for use in ELISA, EMSA, FUNC & IC.
Key Applications
  • ELISA
  • Electrophoretic Mobility Shift Assay
  • Affects Function
  • Immunocytochemistry
Application NotesImmunocytochemistry

ELISA

Stimulation/Inhibition of biological activity

Electrophoretic mobility shift analysis

Optimal working dilutions must be determined by end user.
Biological Information
ImmunogenSynthetic peptide corresponding to amino acids 439-458 of the cytoplasmic tail of human CD4.
Epitopeintracellular
Clone024-10D6.B3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityRecognizes the cytoplasmic tail of CD4. Many functions have been assigned to this tail, including anti-viral regulation (down/up regulation of CD4), T-cell maturation, activation and differentiation, and Lck signal transduction, among others. This antibody is useful as a control for studies involving targeting of intracellular protein domains of T cells.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD4
  • CD4mut
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01730 # Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.
SIZE: 458 amino acids; 51111 Da
SUBUNIT: Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
PTM: Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.
SIMILARITY: SwissProt: P01730 ## Contains 3 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 Ig-like V-type (immunoglobulin-like) domain.
MISCELLANEOUS: Primary receptor for HIV-1.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MAB3706 04053252612534

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Alternative Packsize

Catalogue Number Description
MAB3706-KC Anti-CD4, intracellular, clone 024-10D6.B3 Pur (1mg) KC

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies