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MABF3244-25UG Anti-CD226/DNAM-1 Antibody, clone 10E5

This rat monoclonal Anti-CD226/DNAM-1, clone 10E5, Cat. No. MABF3244 is tested for use in Flow Cytometry and Inhibition, for the detection of CD226/DNAM-1.

NewMABF3244-25UG
25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF3244-25UG
DescriptionAnti-CD226/DNAM-1 Antibody, clone 10E5
Alternate Names
  • DNAX accessory molecule 1
  • DNAM-1
  • PTA1
  • TLiSA1
Background InformationCD226 antigen (UniProt: Q5DW69; also known as Dnam-1, DNAX accessory molecule-1) is encoded by the Cd226 gene (Gene ID: 225825; also known as Dnam-1) in mouse. CD226 is a cell surface glycoprotein that belongs to the immunoglobulin superfamily. It contains 2 Ig-like C2-type domains (aa 19-126 and 297-316) and a transmembrane domain (aa 255-275). It is primarily expressed on the surface of natural killer (NK) cells, T cells, monocytes, and platelets. CD226 plays a crucial role in the regulation of immune responses, particularly in the context of cell-mediated cytotoxicity and immune surveillance. CD226 is involved in the modulation of T cell responses and the regulation of immune tolerance. Dysregulation of CD226 has been implicated in various autoimmune diseases, lupus and cancer, making it an important target for immunotherapy research and treatment development. (Ref.: Huang, Z., et al., (2020). Front. Cell Dev. Biol. 24; Article 564).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in PBS without azide.
Quality LevelMQ200
Applications
ApplicationThis rat monoclonal Anti-CD226/DNAM-1, clone 10E5, Cat. No. MABF3244 is tested for use in Flow Cytometry and Inhibition, for the detection of CD226/DNAM-1.
Key Applications
  • Flow Cytometry
  • Inhibition
Application NotesTested Applications

Inhibition: A representative lot inhibited Th1 cell expansion and in the induction of APCs that inhibit T cell activation. (Dardalhon, V., et al. (2005). J Immunol. 175(3);1558-65).

Flow Cytometry Analysis: A representative lot detected CD226/DNAM-1 in Flow Cytometry applications (Dardalhon, V., et al. (2005). J Immunol. 175(3);1558-65; Fang, L., et al. (2009). J Immunol. 182(9):5453-60; Zhang, Z., et al. (2015). J Exp Med. 212(12):2165-82).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenTh1-polarized T cell clones (AE7).
EpitopeExtracellular domain
Clone10E5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone 10E5 is a rat monoclonal antibody that detects CD226/DNAM-1. It targets an epitope within the extracellular domain of the protein.
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cd226
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight 38.06 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD226/DNAM-1 in C57BL/6 mouse splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3244-25UG 04065271266599