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MABF2812-100UG Anti-CD137 Antibody, clone LOB12.0

Anti-CD137, clone LOB12.0, Cat. No. MABF2812, is a rat monoclonal antibody that detects CD137 and is tested for use in and Flow Cytometry, Competition Assay, Agonist Activity, and Surface plasmon resonance.

MABF2812-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2812-100UG
DescriptionAnti-CD137 Antibody, clone LOB12.0
Alternate Names
  • Tumor necrosis factor receptor superfamily member 9
  • 4-1BB ligand receptor
  • T-cell antigen 4-1BB
Background InformationTumor necrosis factor receptor superfamily member 9 (UniProt: P20334; also known as 4-1BB ligand receptor, T-cell antigen 4-1BB, CD137) is encoded by the Tnfrsf9 (also known as Cd137, IIa, Ly63) gene (Gene ID: 21942) in murine species. CD137 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-23), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 24-187), a transmembrane domain (aa 187-208), and a cytoplasmic domain (aa 209-256). It is a member of the TNFR superfamily that serves as an activation-induced costimulatory molecule. Its expression is observed on activated T cells, NK cells, dendritic cells, eosinophils, mast cells, endothelial cells, and some tumor cells. It is rapidly expressed on activated CD8+ T cells and at lower levels on activated CD4+ T cells. Induction of CD137 expression on CD8+ T cells is reported to be highly sensitive to low levels of TCR stimulation. Engagement of CD137 on T cells stimulates their proliferation independently of CD28 and promotes IL-2 and IFN- secretion by Th effector cells and CTLs, respectively. Expression of CD137 is shown to negatively correlate with lymphocyte proliferation and positively with the degree of activation-induced cell death caused by mitogen overstimulation. Treg cells that infiltrate tumors are reported to express higher levels of CD137 and administration of clone LOB12.0 with agonistic function promotes T cell agonism to promote tumor rejection. (Ref.: Buchan, SL., et al. (2018). Immunity 49(5), 958-970; Taraban, VY., et al. (2002). Eur. J. Immunol. 32(12); 3617-3627).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-CD137, clone LOB12.0, Cat. No. MABF2812, is a rat monoclonal antibody that detects CD137 and is tested for use in and Flow Cytometry, Competition Assay, Agonist Activity, and Surface plasmon resonance.
Key Applications
  • Surface plasmon resonance
  • Competition Assay
  • Agonist or Inhibitor
  • Flow Cytometry
Application NotesTested Applications

Surface plasmon resonance: A representative lot detected CD137 in Surface plasmon resonance applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: 10 µg from a representative lot detected CD137 in Jurkat cells expressing mouse CD137.

Agonist Activity: A representative lot of this antibody co-stimulated CD8+ T cells and potentiated anti-tumor immunity in mice.(Taraban, V.Y., et al. (2002). Eur J Immunol. 32(12); 3617-3627 ).

Competition Assay: A representative lot of this antibody competed for binding to 4-1BB on effector T cells. (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Flow Cytometry Analysis: A representative lot detected CD137 in Flow Cytometry applications (Buchan, S.L., et al. (2018). Immunity. 49(5):958-970.e.7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenMouse CD137 human Fc fusion protein
EpitopeUnknown
CloneLOB12.0
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone LOB12.0 is a rat monoclonal antibody that detects CD137 (Tnfrsf9).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Tnfrsf9
Purification MethodProtein G purified
UniProt Number
Molecular Weight27.6 kDa calculated..
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity confirmation by Isotyping test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be rat IgG2a .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2812-100UG 04065270647207