Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

AP1169 Anti-CCL7 Mouse mAb (4B5)

AP1169
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the CCL7 protein.
Catalogue NumberAP1169
Brand Family Calbiochem®
SynonymsAnti-Monocyte Chemotactic Protein 3, Anti-Small Inducible Cytokine A7, Anti-Chemokine Ligand 7
Application Data
Detection of human CCL7 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with partial recombinant, human CCL7 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL7 Mouse mAb (4B5) (Cat. No. AP1169) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids of 24-100 human CCL7, expressed as a GST fusion protein
Clone4B5
HostMouse
IsotypeIgG2b
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AP1169 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-October-2010 RFH
SynonymsAnti-Monocyte Chemotactic Protein 3, Anti-Small Inducible Cytokine A7, Anti-Chemokine Ligand 7
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human CCL7 by immunoblotting. Sample: Whole cell lysate from 293T cells, transfected with partial recombinant, human CCL7 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-CCL7 Mouse mAb (4B5) (Cat. No. AP1169) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the CCL7 protein.
BackgroundCCL7 is a monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids of 24-100 human CCL7, expressed as a GST fusion protein
Clone4B5
IsotypeIgG2b
Specieshuman
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review