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AB5613 Anti-Brain Derived Neurotrophic Factor Antibody, pro

AB5613
50 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIHC, WBRbSerumPolyclonal Antibody
Description
Catalogue NumberAB5613
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Brain Derived Neurotrophic Factor Antibody, pro
Alternate Names
  • Prepro-Abrineurin
  • Pro-Abrineurin
  • Prepro-BDNF
  • Pro-BDNF
  • Prepro-Brain-derived neurotrophic factor
  • Pro-Brain-derived neurotrophic factor
Background InformationBrain-derived neurotrophic factor (UniProt P23560; also known as Abrineurin, BDNF, Neurotrophin) is encoded by the BDNF (also known as BULN2) gene (Gene ID 627) in human. BDNF is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT3, and NT4/5. BDNF mediates its neurotrophic properties by signaling through the high affinity cell surface receptor gp145/trkB. BDNF is initially produced with a signal peptide (a.a. 1-18) and a propeptide (a.a. 19-128) sequence, the removal of which yields the mature BDNF (a.a. 129-247).
Product Information
FormatSerum
PresentationRabbit serum. Lyophilized. Reconstitute with 50 μL of sterile distilled water.
Quality LevelMQ100
Applications
ApplicationAnti-Brain Derived Neurotrophic Factor Antibody, pro is an antibody proBDNF for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:1,000-1:5,000

Immunohistochemistry: 1:1,000-1:5,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region within human BDNF propeptide sequence.
EpitopePropeptide.
HostRabbit
SpecificityThis polyclonal antiserum detects prepro- and pro-BDNF, as well as prepro-/pro-BDNF-derived N-terminal fragments that contain the propeptide sequence (a.a. 19-128), but not mature BDNF or C-terminal fragments without the propeptide sequence. No cross-reactivity to other proneurotrophins.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • BDNF
UniProt Number
Molecular Weight~35 kDa (glycosylated prepro-/pro-BDNF), ~50-65 kDa (glycosylated prepro-/pro-BDNF dimer), and ~26-28 kDa (non-glycosylated prepro/pro-BDNF or glycosylated prepro-/pro-BDNF N-terminal fragments) observed. Target bands appear larger than the calculated molecular weights of 27.82/28.87/29.82/37.12/31.12 kDa (human prepro-BDNF isoform 1/2/3/4/5) and 25.77/26.82/27.77/35.07/29.06 kDa (human pro-BDNF isoform 1/2/3/4/5) due to glycosylation and/or dimerization. Uncharacterized band(s) may appear in some lysates.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain at -20°C undiluted for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (ACS grade or better) can be added (1:1) for additional stability.
Packaging Information
Material Size50 µL
Global Trade Item Number
Catalogue Number GTIN
AB5613 04053252274855