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05-449-AF488 Anti-Bin1 Antibody, clone 99D Antibody, Alexa Fluor® 488 Conjugate

Detect Bin1 using this mouse monoclonal Anti-Bin1 antibody, clone 99D, Alexa Fluor® 488 Conjugate, Cat. No. 05-449-AF488, validated for use in Flow Cytometry and Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Chicken, Rat, Human, Mouse
05-449-AF488
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number05-449-AF488
DescriptionAnti-Bin1 Antibody, clone 99D Antibody, Alexa Fluor® 488 Conjugate
Alternate Names
  • Amphiphysin II
  • Amphiphysin-like protein
  • Box-dependent myc-interacting protein 1
  • Bridging integrator 1
Background InformationMyc box-dependent-interacting protein 1 (UniProt: O00499; also known as Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1, BIN1) is encoded by the BIN1 (also known as AMPHL) gene (Gene ID: 274) in human. BIN1 is expressed in many tissues, but has the highest expression in brain and muscle. In brain it is concentrated in the cytomatrix beneath the plasma membrane of axon initial segments and nodes of Ranvier. In skeletal muscle, it is concentrated around the plasmalemma of T tubules. BIN1 is involved in regulation of synaptic vesicle endocytosis and is also reported to act as a tumor suppressor that inhibits malignant cell transformation. BIN1 is considered as a dominant autoantigen in paraneoplastic Stiff-man syndrome. Mutations in BIN1 gene are known to cause myopathy centronuclear 2 (CNM2), a congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. Ref.: Butler, MH., et al., (1997). J. Cell Biol. 137(6): 1355 1367.
References
Product Information
FormatAlexaFluor®488
PresentationPurified mouse monoclonal antibody IgG2b in PBS with 1.5% BSA and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Bin1 using this mouse monoclonal Anti-Bin1 antibody, clone 99D, Alexa Fluor® 488 Conjugate, Cat. No. 05-449-AF488, validated for use in Flow Cytometry and Immunocytochemistry.
Key Applications
  • Immunocytochemistry
  • Flow Cytometry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bin1 in HeLa cells.

Flow Cytometry Analysis: A 1:400 dilution from a representative lot detected Bin1 in one million HeLa cells.
Biological Information
ImmunogenLinear peptide corresponding to human Bin1.
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 99D, Alexa Fluor® 488 Conjugate detects Bin1 in A431 and HeLa cells by immunocytochemistry.
IsotypeIgG2bκ
Species Reactivity
  • Chicken
  • Rat
  • Human
  • Mouse
Species Reactivity NoteChicken, Human, Mouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BIN1
  • AMPHL
Purification MethodProtein G purified
UniProt Number
Molecular Weight64.7 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Bin1 in A431 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
05-449-AF488 04054839139130