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MABC1176 Anti-Bax Antibody, clone 6A7

Anti-Bax, clone 6A7, Cat. No. MABC1176, is a mouse monoclonal antibody that detects Bax and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Bax Antibody, clone 6A7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: BAX, BCL2L4 UniProt Number: Q07812
MABC1176
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIF, IP, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1176
DescriptionAnti-Bax Antibody, clone 6A7
Alternate Names
  • Apoptosis regulator BAX
  • Bcl-2-like protein 4
  • Bcl2-L-4
Background InformationApoptosis regulator Bax (UniProt: Q07812; also known as Bcl-2-like protein 4, Bcl2-L-4) is encoded by the BAX (also known as BCL2L4) gene (Gene ID: 581) in human. Bax is a pro-apoptotic member of the Bcl-2 family of proteins. It displays extensive homology with Bcl-2 and is variably expressed in different cells, usually at very low concentrations in normal cells. Upon induction of apoptosis Bax undergoes a conformational change and translocates from cytosol to mitochondrial membrane. Bax forms homodimers as well as heterodimers with Bcl-2 through common binding motif and the ratio of Bax to Bcl-2 determines the survival or death of cells during apoptosis. BAX is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. Clone 6A7 reacts with multiple isoforms of Bax.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Bax, clone 6A7, Cat. No. MABC1176, is a mouse monoclonal antibody that detects Bax and has been tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Bax in Western Blotting applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunofluorescence Analysis: A representative lot detected Bax in Immunofluorescence applications (Xin, M., et. al. (2014). Nat Commun. 5:4935).

Immunoprecipitation Analysis:A representative lot detected Bax in Immunoprecipitation applications (Xin, M., et. al. (2014). Nat Commun. 5:4935; Hsu, Y.T., et. al. (1998). J Biol Chem. 273(17):10777-83).
Biological Information
ImmunogenKLH-cojugated linear peptide coprresponding to 13 amino acids from the N-terminal region of human BAX.
Clone6A7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 6A7 recognizes BAX in human cells. It targets an epitope with in 13 amino acids from the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BAX
  • BCL2L4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~15 kDa observed; 21.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s). Multiple isoforms for this target (8), ranging in MWs between 4-24 kDa, are reported. Most of the predicted MWs reside between 12-19 kDa. Based on the sequence it is expected to react with isoforms 1,2,4,5, and 8.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunoprecipitation in THP-1 cell lysates.

Immunoprecipitation Analysis: A 1:250 dilution of this antibody detected Bax in THP-1 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1176 04054839322495