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MABF2319-25UG Anti-BLAST-2 (CD23) Antibody, clone EBVCS4

Anti-BLAST-2 (CD23), clone EBVCS4, Cat. No. MABF2319, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tesed for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Radioimmunoassay.

MABF2319-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2319-25UG
DescriptionAnti-BLAST-2 (CD23) Antibody, clone EBVCS4
Alternate Names
  • Low affinity immunoglobulin epsilon Fc receptor
  • C-type lectin domain family 4 member J
  • CD23
  • Fc-epsilon-RII
  • Immunoglobulin E-binding factor
  • Lymphocyte IgE receptor
Background InformationLow affinity immunoglobulin epsilon Fc receptor (UniProt: P06734; also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23) is encoded by the FCER2 (also known as CD23A, CLEC4J, FCE2, IGEBF) gene (Gene ID: 2208) in human. Epstein-Barr virus (EBV) is reported to transform human B lymphocytes and is a causative agent for Burkitt s lymphoma and infectious mononucleosis. EBV induces the expression of one or more antigens (e.g., CD23) on the surface of adult human B-lymphocytes soon after infection. CD23 is a homotrimeric, single-pass type II membrane protein that serves as a low-affinity receptor for Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in the differentiation of B-cells. CD23 can also exist as a soluble excreted form, sCD23 (aa 150-321), which is produced by ADAM10-mediated ectoderm shedding. CD23 has a cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-47), and an extracellular domain (aa 48-321). Clone EBVCS4 can specifically detect antigens that are present on the surface of EBV-transformed cells. These antigens are also recognized by autochthonous cytotoxic T cells specific for EBV-transformed cells. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
Applications
ApplicationAnti-BLAST-2 (CD23), clone EBVCS4, Cat. No. MABF2319, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tesed for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Radioimmunoassay.
Key Applications
  • Flow Cytometry
  • Immunofluorescence
  • Immunoprecipitation
  • Radioimmunoassay
Application NotesRadioimmunoassay Analysis: A representative lot detected BLAST-2 (CD23) in Radioimmunoassay applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).

Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).

Immunoprecipitation Analysis: A representative lot immunoprecipitated BLAST-2 (CD23) in Immunoprecipitation applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Biological Information
ImmunogenEBV-transformed human cells.
CloneEBVCS4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone EBVCS4 is a mouse monoclonal antibody that specifically detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) in human cells.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • FCER2
  • CD23A
  • CLEC4J
  • FCE2
  • IGEBF
Purification MethodLigatrap
UniProt Number
Molecular Weight36.47 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Raji B cells.

Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in one million Raji cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2319-25UG 04054839010552

Documentation

Anti-BLAST-2 (CD23) Antibody, clone EBVCS4 SDS

Title

Safety Data Sheet (SDS) 

Anti-BLAST-2 (CD23) Antibody, clone EBVCS4 Certificates of Analysis

TitleLot Number
Anti-BLAST-2 (CD23), clone EBVCS4 - 3253076 3253076

References

Reference overviewPub Med ID
Characterization of an antigen whose cell surface expression is induced by infection with Epstein-Barr virus.
Sugden, B; Metzenberg, S
J Virol  46  800-7  1983

Show Abstract 		6304342 6304342
Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr virus.
Kintner, C; Sugden, B
Nature  294  458-60  1981

Show Abstract 		6273741 6273741