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OP191 Anti-BCRP Mouse mAb (BXP-21)

Anti-BCRP Mouse mAb (BXP-21) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP191
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~70 kDa BCRP protein in VP cells.
Catalogue NumberOP191
Brand Family Calbiochem®
Application Data
Detection of human BCRP by immunocytochemistry. Samples: Parental IGROV1 ovarian tumor cell line (figure A) and the drug-selected BCRP-overexpressing subline T8 (figure B). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:50). Detection: fluorescence.

Detection of human BCRP by immunohistochemistry. Samples: Placenta (figure A), liver (figure B), small intestine (figure C), colon (figure D), prostate (figure E), ovary (figure F), submucosa of the stomach (figure G), and choroid plexus (figure H) with anti-BCRP clone BXP-21 (figures D and H) or BXP-34 (figures A-C, E-G). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:20). Detection: chemiluminescence.
References
ReferencesScheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.
Product Information
FormLiquid
FormulationCulture supernatant, 0.7% BSA.
Positive controlMCF7/AdrVP cells
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunocytochemistry
Paraffin Sections
Application NotesFrozen Sections (1:20)
Immunoblotting (1:50)
Immunocytochemistry (1:20-1:50)
Paraffin Sections (1:20, heat pre-treatment required)
Application CommentsDoes not cross-react with human MDR1, MRP1, or MRP2. Formaldehyde-fixed, paraffin-embedded tissues require pre-treatment with 10 mM citric acid, pH 6.0, at 95°C for 20 min. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 271-396 of human breast cancer resistance protein (BCRP) fused to maltose-binding protein
ImmunogenHuman
CloneBXP-21
HostMouse
IsotypeIgG2a
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP191 0

Documentation

Anti-BCRP Mouse mAb (BXP-21) SDS

Title

Safety Data Sheet (SDS) 

Anti-BCRP Mouse mAb (BXP-21) Certificates of Analysis

TitleLot Number
OP191

References

Reference overview
Scheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationFrozen Sections (1:20)
Immunoblotting (1:50)
Immunocytochemistry (1:20-1:50)
Paraffin Sections (1:20, heat pre-treatment required)
Application Data
Detection of human BCRP by immunocytochemistry. Samples: Parental IGROV1 ovarian tumor cell line (figure A) and the drug-selected BCRP-overexpressing subline T8 (figure B). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:50). Detection: fluorescence.

Detection of human BCRP by immunohistochemistry. Samples: Placenta (figure A), liver (figure B), small intestine (figure C), colon (figure D), prostate (figure E), ovary (figure F), submucosa of the stomach (figure G), and choroid plexus (figure H) with anti-BCRP clone BXP-21 (figures D and H) or BXP-34 (figures A-C, E-G). Primary antibody: Anti-BCRP Mouse mAb (BXP-21) (Cat. No. OP191) (1:20). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody supplied as culture supernatant. Recognizes the ~70 kDa BCRP.
BackgroundBreast cancer resistance protein (BCRP) is a transmembrane half-transporter that plays a major role in the multidrug resistance phenotype of human breast cancer. Overexpression of BCRP actively reduces the intracellular drug concentration of some chemotherapeutics and their efficacy.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of amino acids 271-396 of human breast cancer resistance protein (BCRP) fused to maltose-binding protein
CloneBXP-21
IsotypeIgG2a
Specieshuman
Positive controlMCF7/AdrVP cells
FormLiquid
FormulationCulture supernatant, 0.7% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with human MDR1, MRP1, or MRP2. Formaldehyde-fixed, paraffin-embedded tissues require pre-treatment with 10 mM citric acid, pH 6.0, at 95°C for 20 min. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesScheffer, G.L., et al. 2000. Cancer Res. 60, 2589.
Doyle, L.A., et al. 1998. Proc. Natl. Acad, Sci. USA 95, 15665.