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MABN2527-25UG Anti-Aquaporin 4 Antibody, clone E5415A

Anti-Aquaporin 4, clone E5415A, Cat. No. MABN2527, is a mouse monoclonal antibody that detects Aquapoorin-4 and is tested for use in ELISA, Function Analysis, Immunocytochemistry, Immunofluorescence,

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MABN2527-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABN2527-25UG
DescriptionAnti-Aquaporin 4 Antibody, clone E5415A
Alternate Names
  • AQP-4
  • Mercurial-insensitive water channel
  • MIWC
  • WCH4
Background InformationAquaporin-4 (UniProt: P55087; also known as AQP-4, Mercurial-insensitive water channel, MIWC, WCH4) is encoded by the AQP4 gene (Gene ID: 361) in human. Aquaporin-4 is a multi-pass membrane protein that forms a water-specific channel and serves as an osmoreceptor that regulates body water balance and mediates water flow within the central nervous system. Its extracellular domains are localized to amino acids 58-64, 137-155, and 206-231); its transmembrane domains are in amino acids 37-57, 65-85, 116-136, 156-176, 185-205, and 232-252); and the cytoplasmic domain is in amino acids 1-36, 86-115, 177-184, and 253-323). Aquaporin-4 is largely expressed in the brain and the skeletal muscle and at lower levels in heart, kidney, and lung tissue. Phosphorylation of Aquaporin-4 at Serine 180 by protein kinase C is shown to reduce conductance by about 50%, whereas phosphorylation at Serine 111 by protein kinase G in response to glutamate can increase conductance by about 40%. Two isoforms of Aquaporin-4 have been described that are produced by alternative splicing. Antibodies to Aquaporin-4 can serve as useful serum markers to distinguishing neuromyelitis Optica (NM) from multiple sclerosis. Clone mCCD does not cause redistribution of Aquaporin-4 and also it does not affect coaggregation astrocytic CD32-B and Aquaporin-4. (Ref.: Hinson, SR et al. (2017). Proc. Natl. Acad. Sci. USA 114(21); 5491-5496; Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Aquaporin 4, clone E5415A, Cat. No. MABN2527, is a mouse monoclonal antibody that detects Aquapoorin-4 and is tested for use in ELISA, Function Analysis, Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • ELISA
  • Affects Function
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Application NotesFunction Analysis: A representative lot caused a loss of AQP4 in an experimental autoimmune encephalomyelitis (EAE) rat model in a dose dependent manner and enhanced endocytosis of endogenous AQP4 in astrocytes. (Kurosawa, K., et. al. (2015). Acta Neuropathol Commun. 3:82; H uang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83) .

ELISA Analysis: A representative lot detected Aquaporin 4 in ELISA applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aquaporin 4 in mouse astrocytes.

Western Blotting Analysis: A representative lot detected Aquaporin 4 in Western Blotting applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Immunofluorescence Analysis: A representative lot detected Aquaporin 4 in Immunofluorescence applications (Huang, P., et. al. (2016). Biochem Biophys Rep. 7:77-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenRecombinant extracellular domain of murine Aquaporin 4.
EpitopeExtracellular domain
CloneE5415A
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone E5415A is a mouse monoclonal antibody generated in AQP-4 knockout mice that detects murine Aquaporin-4. It targets an epitope within the extracellular domain. It detects both M1 (Isoform 2) and M23 (Isoform 1).
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteMouse, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Aqp4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~45 kDa observed; 34.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Mouse heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Aquaporin 4 in Mouse heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Global Trade Item Number
Catalogue Number GTIN
MABN2527-25UG 04065265872539