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ABE552 Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody

This Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: FTO UniProt Number: Q9C0B1
ABE552
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE552
DescriptionAnti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody
Alternate Names
  • Alpha-ketoglutarate-dependent dioxygenase FTO
  • Fat mass and obesity-associated protein
Background InformationAlpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Alpha-ketoglutarate-dependent dioxygenase FTO Antibody is validated for use in western blotting & IP for the detection of Alpha-ketoglutarate-dependent dioxygenase FTO.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated Alpha-ketoglutarate-dependent dioxygenase FTO from 500 μg of U2OS nuclear extract.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Alpha-ketoglutarate-dependent dioxygenase FTO.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Chimpanzee, Monkey, Ox, and Sheep based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • FTO
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~58 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in U2OS nuclear extract.

Western Blotting Analysis: 1 μg/mL of this antibody detected Alpha-ketoglutarate-dependent dioxygenase FTO in 10 μg of U2OS nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE552 04053252945687