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MABS2267-100UL Anti-ANT1/SLC25A4 Antibody, clone IF3F11

Anti-ANT1/SLC25A4, clone IF3F11, Cat. No. MABS2267, is a mouse monoclonal antibody that detects ANT1/SLC25A4 and is tested for use in Western Blotting.

MABS2267-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABS2267-100UL
DescriptionAnti-ANT1/SLC25A4 Antibody, clone IF3F11
Alternate Names
  • ADP/ATP translocase 1
  • ADP/ATP carrier protein 1
  • ADP/ATP carrier protein
  • heart/skeletal muscle isoform T1
  • Adenine nucleotide translocator 1
  • Solute carrier family 25 member 4
Background InformationADP/ATP translocase 1 (UniProt: P12235; also known as ADP/ATP carrier protein 1, ADP/ATP carrier protein, heart/skeletal muscle isoform T1 1 , Adenine nucleotide translocator 1, ANT 1, Solute carrier family 25 member 4) is encoded by the SLC25A4 (also known as AAC1, ANT1) gene (Gene ID: 291) in human. ANT1 is a multi-pass inner mitochondrial membrane protein with six transmembrane domains. It serves as an ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to provide energy for cellular functions. It is reported to cycle between the cytoplasmic-open state (c-state) and the matrix-open state (m-state) and operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic or matrix side of the inner mitochondrial membrane. It is also involved in mitochondrial uncoupling and mitochondrial permeability transition pore activity. ANT also acts as a regulator of mitophagy independently of ADP:ATP antiporter activity. It promotes mitophagy via interaction with TIMM44 that leads to inhibition of presequence translocase TIMM23, thereby promoting stabilization of PINK1. ANT1 deficiency has been implicated in various pathological states, including hypertrophic cardiomyopathy, mitochondrial myopathy, lactic acidosis, and progressive external opthalmoplegia. (Ref.: Hoshino, A., et al. (2019). Nature. 575(7782); 375-379; Lu, Y-W., et al. (2017). Mol. Biol. Cell. 28(11):1489-1506; Kawamaoto, H., et al. (2011). Hum. Mol. Genet. 20(15); 2964-2974).
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-ANT1/SLC25A4, clone IF3F11, Cat. No. MABS2267, is a mouse monoclonal antibody that detects ANT1/SLC25A4 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesTested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected ANT1/SLC25A4 in Human skeletal muscle and Human brain tissue extracts.

Western Blotting Analysis: A representative lot detected ANT1/SLC25A4 in Western Blotting applications (Lu, Y-W., et al. (2017). Mol Biol Cell. 28(11):1489-1500; Acoba, M.G., et al. (2021). Cell Rep. 34(11):108869).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged, full-length, recombinant human ADP/ATP translocase 1 (ANT1).
EpitopeInternal
CloneIF3F11
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone IF3F11 is a mouse monoclonal antibody that detects Adenine nucleotide translocator 1 (ANT1/SLC25A4). It targets an epitope within 15 amino acids from the internal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SLC25A4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~33 kDa observed; 33.06 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Human heart tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ANT1/SLC25A4 in Human heart tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MABS2267-100UL 04065270321633